Are the MicroAmp™ Optical 96-Well Reaction Plates and MicroAmp™ Optical 384 -Well Reaction Plates autoclavable?

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Answer

Our microplates are certified to be DNA, RNase and PCR inhibitor free as long as they are kept in the original packaging bag. They are not meant to be autoclaved.

Answer Id: 2500

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Can I place a standard MicroAmp® 96-Well Optical Reaction Plate onto a 7500 Fast Real-Time PCR System?

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No. The plate does not fit and will result in the crushing of the wells and possible contamination of the block due to leaking of the well contents.

Answer Id: 2356

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What is the difference between the MicroAmp® Optical Adhesive Covers and the MicroAmp® Optical Caps? Can they be used interchangeably?

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Answer

The MicroAmp® Optical Adhesive Covers (P/N 4311971) have a higher optical efficiency than the MicroAmp® Optical Caps, (8 caps/strip) (P/N 4323032). They can be used instead of the MicroAmp® Optical Caps only when using the MicroAmp® 96-Well Reaction Plate with Barcode (P/N 4306737, 4309849) or the MicroAmp® Optical 96-Well Reaction Plate (P/N N801-0560). The Optical Adhesive Covers cannot be used with the MicroAmp® Optical Tubes.

Answer Id: 1366

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What is the recommended minimum and maximum volume that can be used on any Applied Biosystems® Real-Time PCR Instrument?

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Answer

The reaction volume per well is depends on what type of plate of a particular instrument that you use. The answer is provided by the information below based on the plate type.

96-well standard plate (Part Numbers: N8010560, 4316813, 4306737 and 4326659) Recommend volume: 50ul; Maximum volume: 100ul; Minimum volume: 25ul

96-well fast plate (Part Numbers: 4346907, 4346906 or 4366932) Recommend volume: 20ul; Maximum volume: 30ul; Minimum volume: 10ul

48-well fast plate (Part Numbers: 4375816) Recommend volume: 20ul; Maximum volume: 30ul; Minimum volume: 10ul

384-well plate (Part Numbers: 4309849, 4326720, 4343814 and 4343370) Recommended volume: 10ul; Maximum volume: 30ul; Minimum volume: 5ul

Answer Id: 2742

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