Can Oligo d(T)16 RT primers be used with the TaqMan™ Human Endogenous Control Plate (P/N 4309199)?
No. The reverse transcription of total RNA to cDNA must be done with random hexamers or random primers (or a mixture of random and oligo dT primers) because the 18S rRNA assay cannot evaluate poly A+ purified RNA samples as most of the ribosomal RNA has been removed. The plate has been designed to evaluate only total RNA.
Answer Id: E1360
Was this answer helpful?