Can Oligo d(T)16 RT primers be used with the TaqMan® Human Endogenous Control Plate (P/N 4309199)?

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Answer

No. The reverse transcription of total RNA to cDNA must be done with random hexamers or random primers (or a mixture of random and oligo dT primers) because the 18S rRNA assay cannot evaluate poly A+ purified RNA samples as most of the ribosomal RNA has been removed. The plate has been designed to evaluate only total RNA.

Answer Id: E1360

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