The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability.

Citations & References

  • Authors: Niesen FH, Berglund H, Vedadi M,
  • Journal: Nat Protoc (2007) 2:2212-2221
  • Product Usage: Identification of protein-binding ligands via fluorescence monitoring of thermal unfolding characteristics.
  • ID: PN67268
Catalog #s
A47
B153
D12800
N1142
S6650
S6651
cbda885a3b59a6c7bb0ba1de69344a3f_Citations

Methods for protein characterization by mass spectrometry, thermal shift (ThermoFluor) assay, and multiangle or static light scattering.

Citations & References

  • Authors: Nettleship JE, Brown J, Groves MR, Geerlof A,
  • Journal: Methods Mol Biol (2008) 426:299-318
  • Product Usage: High-throughput fluorescence-based monitoring of protein thermal stability and its conditioning by buffers, additives and ligands. The measurements are performed on a real-time PCR analyzer.
  • ID: PN67274
Catalog #s
S6650
S6651
9eae1dd75c45bd2bbff49640b2a918bf_Citations

High throughput methods of assessing protein stability and aggregation.

Citations & References

  • Authors: Senisterra GA, Finerty PJ,
  • Journal: Mol Biosyst (2009) 5:217-223
  • Product Usage: Review comparing differential scanning fluorimetry (DSF) using SYPRO Orange dye, differential static light scattering (DSLS), and isothermal denaturation (ITD) for analysis of protein stability and aggregation.
  • ID: PN67320
Catalog #s
S6650
S6651
161685e0a47201788467198c95e512e2_Citations

High-density miniaturized thermal shift assays as a general strategy for drug discovery.

Citations & References

  • Authors: Pantoliano MW, Petrella EC, Kwasnoski JD, Lobanov VS, Myslik J, Graf E, Carver T, Asel E, Springer BA, Lane P, Salemme FR
  • Journal: J Biomol Screen (2001) 6:429-440
  • Product Usage:
  • ID: PN46928
Catalog #s
A47
A50
D12800
T53
181b5acb3e3cd0ced62c3a3dde14c684_Citations