Intensity calibration and automated cell cycle gating for high-throughput image-based siRNA screens of mammalian cells.
Citations & References
- Authors: Poon SS, Wong JT, Saunders DN, Ma QC, McKinney S, Fee J, Aparicio SA,
- Journal: Cytometry A (2008) 73:904-917
- Product Usage: (1) Estimating the global background and signal response (InSpeck) and calibration of spatial illumination (TetraSpeck) for high content microscopy screening (HCMS). (2) Cell cycle analysis (DAPI/BrdU). (3) siRNA transfection (Lipofectamine 2000).
- ID: PN67479