Can I use the original Quant-iT™ Kits with the Qubit® Fluorometer?

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Answer

No, we do not recommend this. Some of the dyes in the original Quant-iT™ kits (those NOT listed as “for use with the Qubit® fluorometer”) are not compatible with the Qubit® Fluorometer. In addition, the new Quant-iT™ kits (labeled as “for use with the Qubit® Fluorometer”) have standards formulated to be more accurate with the Qubit® fluorometer. The Qubit® Fluorometer-compatible kits are also less expensive per assay if you are processing fewer than 20 samples at a time.

Answer Id: E5015

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550941aaebd324d688c53937dbb56f78_FAQ

Can I use the Quant-iT™ and Qubit® Kits with other fluorometers?

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Answer

All Quant-iT™ and Qubit® kits are compatible with all fluorometers and microplate readers that have the appropriate light sources and filters. You won’t have access to the mathematical algorithm in the Qubit® kit for generating the most accurate standard curve, so you just want to make a few dilutions of the highest standard curve in the kit so you can generate a curve from more points. This will give you better accuracy on another fluorometer.

Answer Id: E5016

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6d41993e0e849876c60460cbceeff265_FAQ

Can I use my old Quant-iT™ Kits labeled “for use with Qubit® Fluorometer” with the Qubit® 2.0 Fluorometer?

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Answer

Yes, these kits will work with both the original Qubit® Fluorometer and the Qubit® 2.0 Fluorometer.

Answer Id: E8117

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2bcdf2e10d3d2fce644f4228f8742971_FAQ

I have a crude lysate. Will the Quant-iT® and Qubit® assays work?

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Answer

Generally, the cleaner the sample the better. Some salts, proteins, and detergents are tolerated in the assays; see the specific assay protocol for which ones and at what concentrations.

Answer Id: E8121

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139b86a8693bd7f3933ab6dfcbb3a2bd_FAQ

Can I make my own assay for the Qubit® Fluorometer?

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Answer

Yes, you can, for Qubit® instruments developed after the original Qubit® (1.0) Fluorometer. See MyQubit assay instructions here (http://www.lifetechnologies.com/us/en/home/life-science/laboratory-instruments/fluorometers/qubit/qubit-assays/myqubit.html.html).

Answer Id: E8122

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4e11284ffea58b27ab8a2a150976ce81_FAQ

What is the difference between the Quant-iT™ PicoGreen® DNA, Quant-iT™ DNA, and Qubit® DNA assays?

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Answer

The Quant-iT™ PicoGreen® DNA assay is the oldest assay and the most general-purpose. It requires the most user preparation and calculation, but can be adapted for cuvettes or plates. The Quant-iT™ DNA Assays are designed for high throughput (>20 samples) and for use in 96-well plate readers with no further adaptation. The Qubit® assays are intended for low throughput (<20 samples), and are only used on the Qubit® Fluorometer. The Qubit® Fluorometer contains highly optimized algorithms that calculate the concentration of your sample for you. The performance of all of these assays is similar.

Answer Id: E8129

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b52feac836c4c47b68d20bd366f3cb59_FAQ

Does DNA length have an effect on the dsDNA assays?

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Answer

Yes, short fragments may not stain well and plasmid DNA results will vary depending on conformation.

Answer Id: E8131

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7eac725bc6ee28c9955cbc3744c5f1e1_FAQ

I’m trying to quantify some DNA labeled with a fluorophore. Will this work?

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Answer

PicoGreen® dye and other fluorescence-based quantification reagents are not recommended for quantifying dye-conjugated nucleic acids. The attached dye molecules can interfere with either binding and/or fluorescence output of the quantification reagents.

Answer Id: E8134

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d8b46f7c108dee9cfe029431c2b0b2e2_FAQ

What is the useful pH range for Quant-iT™ DNA kits?

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Answer

The buffer included in the kit should assure the proper pH range, even if your DNA is at a pH outside of this range, since at least a 10-fold excess of kit buffer over sample is used in the assay.

Answer Id: E8135

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5044e7feee0266ff9cbc68cf9ff484a7_FAQ

I have a Quant-iT™ DNA Kit and want to use it for the Qubit® Fluorometer. Can I?

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Answer

Yes, the manual has directions for this application. You will use the 0 ng/μL lambda dsDNA HS standard to generate Standard #1. You will prepare a dilution of the 10 ng/μL lambda dsDNA HS standard to generate Standard #2. You then prepare the samples and compare them to this 2-point standard curve. The Quant-iT dsDNA BR Kit can be used in a similar manner.

Answer Id: E8136

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d56ba5e63a51054371a5dc718c75734f_FAQ