How can I tell whether or not detergent in a sample can be removed with Prosorb® Sample Preparation Cartridges?

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Answer

If the detergent identity is known, look up its CMC (critical micellar concentration); a table of these is listed in the Biological Detergents section of the Sigma-Aldrich® catalog. If you are above the CMC, the detergent forms micelles that cannot be removed by the filtering membrane, so the sample must be diluted prior to application to the Prosorb® Sample Preparation Cartridges.

Answer Id: 1269

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e00406144c1e7e35240afed70f34166a_FAQ

I need to buy media to prepare agar plates, which I will be using to grow colonies after transformation (molecular cloning). Which product would you recommend?

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Answer

We offer two options:

The first is our formulation of LB agar (catalog number 22700-025), which has LB Media and agar mixed together. This comes in a 500g and 2.5 kg size. All you have to do is measure out, add water, and autoclave.

The second option is even easier. Our imMedia™ comes premeasured in pouches. We have three types: imMedia™ Amp Agar, Kan Agar and Zeo Agar for ampicillin, kanamycin and Zeocin™-containing plates. All you have to do is empty the pouch, add water, heat and pour plates. No autoclaving is needed! Each pouch contains sufficient reagents for 8-10 plates.

imMedia™ Amp Agar, 20 pouches, Q601-20

imMedia™ Kan Agar, 20 pouches, Q611-20

imMedia™ Zeo Agar, 20 pouches, Q621-20

For added convenience, you should also check out our LB medium imMedia™ products: (Each pouch makes 200 mL of media)

imMedia™ Amp Liquid, 20 pouches, Q600-20

imMedia™ Kan Liquid, 20 pouches, Q610-20

imMedia™ Zeo Liquid, 20 pouches, Q620-20

Answer Id: 3039

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f0204e1d3ee3e4b05de4e2ddbd39e076_FAQ

What are the effective concentrations for the antibiotics, IPTG, and X-gal in your imMedia™ products?

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The effective antibiotic concentrations in imMedia™ is: Ampicillin - 100 ug/ml, Kanamycin - 50 ug/ml, Zeocin - 25 ug/ml. Plates for blue/white color selection contain: IPTG - 100 ug/ml, X-gal - 100 ug/ml.

Answer Id: 3678

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2151b4c76b4dcb048d06a5c32942b6f6_FAQ

What is in imMedia™?

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Answer

imMedia™ is supplied in individual ready-to-use pouches. Each pouch contains low-salt LB medium components, with or without agar, and antibiotic (Ampicillin, Kanamycin, or Zeocin™). The imMedia™ Blue products also include IPTG and X-gal for blue/white colony screening. To maintain the activities of heat-sensitive media components (i.e. antibiotics, IPTG, and X-gal) during and after microwave heating, imMedia™ contains special heat stabilizers.

Answer Id: 3679

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74791edf1f8e8b8289a5067737630874_FAQ

Is imMedia™ sterile?

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Answer

Yes. The components in each imMedia™ pouch are pre-sterilized by irradiation. This allows for preparation of sterile medium without autoclaving.

Answer Id: 3680

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2122c699d5e3d2fa6690771845bd7904_FAQ

Do I have to use sterilized water and flasks to prepare imMedia™?

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Answer

No. While sterilized water and flasks are preferred in all medium preparation, we use deionized water and clean, washed flasks in our quality control experiments to test the sterility of imMedia™. The pre-sterilized imMedia™ components, microwaving process, and the presence of antibiotic ensure that the medium maintains sterility during overnight incubation.

Answer Id: 3681

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9426c311e76888b3b2368150cd05f362_FAQ

How do you test imMedia™?

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To ensure the consistency and quality of imMedia™, each lot is tested for sterility, E. coli growth support, and selection efficiency using E. coli strains that carry the appropriate antibiotic resistance marker. imMedia™ must display the same growth support and selection efficiency as conventional LB medium. imMedia™ Blue products are further tested to ensure efficient blue color development. Colonies grown on agar plates prepared with imMedia™ Blue medium must develop a deep blue color within 16 hours of incubation at 37 C.

Answer Id: 3682

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898aef0932f6aaecda27aba8e9903991_FAQ

What is the shelf life of imMedia™?

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Answer

imMedia™ is provided in sealed pouches and should be stored at room temperature. When stored properly, the stability and quality of imMedia™ is guaranteed for six months.

Answer Id: 3683

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