I need to buy media to prepare agar plates, which I will be using to grow colonies after transformation (molecular cloning). Which product would you recommend?
We offer two options:
The first is our formulation of LB agar (catalog number 22700-025), which has LB Media and agar mixed together. This comes in a 500g and 2.5 kg size. All you have to do is measure out, add water, and autoclave.
The second option is even easier. Our imMedia™ comes premeasured in pouches. We have three types: imMedia™ Amp Agar, Kan Agar and Zeo Agar for ampicillin, kanamycin and Zeocin™-containing plates. All you have to do is empty the pouch, add water, heat and pour plates. No autoclaving is needed! Each pouch contains sufficient reagents for 8-10 plates.
imMedia™ Amp Agar, 20 pouches, Q601-20
imMedia™ Kan Agar, 20 pouches, Q611-20
imMedia™ Zeo Agar, 20 pouches, Q621-20
For added convenience, you should also check out our LB medium imMedia™ products: (Each pouch makes 200 mL of media)
imMedia™ Amp Liquid, 20 pouches, Q600-20
imMedia™ Kan Liquid, 20 pouches, Q610-20
imMedia™ Zeo Liquid, 20 pouches, Q620-20
Answer Id: 3039
How can I tell whether or not detergent in a sample can be removed with Prosorb® Sample Preparation Cartridges?
If the detergent identity is known, look up its CMC (critical micellar concentration); a table of these is listed in the Biological Detergents section of the Sigma-Aldrich® catalog. If you are above the CMC, the detergent forms micelles that cannot be removed by the filtering membrane, so the sample must be diluted prior to application to the Prosorb® Sample Preparation Cartridges.
Answer Id: 1269
What are the effective concentrations for the antibiotics, IPTG, and X-gal in your imMedia™ products?
What is in imMedia™?
imMedia™ is supplied in individual ready-to-use pouches. Each pouch contains low-salt LB medium components, with or without agar, and antibiotic (Ampicillin, Kanamycin, or Zeocin™). The imMedia™ Blue products also include IPTG and X-gal for blue/white colony screening. To maintain the activities of heat-sensitive media components (i.e. antibiotics, IPTG, and X-gal) during and after microwave heating, imMedia™ contains special heat stabilizers.
Answer Id: 3679
Is imMedia™ sterile?
Do I have to use sterilized water and flasks to prepare imMedia™?
No. While sterilized water and flasks are preferred in all medium preparation, we use deionized water and clean, washed flasks in our quality control experiments to test the sterility of imMedia™. The pre-sterilized imMedia™ components, microwaving process, and the presence of antibiotic ensure that the medium maintains sterility during overnight incubation.
Answer Id: 3681
How do you test imMedia™?
To ensure the consistency and quality of imMedia™, each lot is tested for sterility, E. coli growth support, and selection efficiency using E. coli strains that carry the appropriate antibiotic resistance marker. imMedia™ must display the same growth support and selection efficiency as conventional LB medium. imMedia™ Blue products are further tested to ensure efficient blue color development. Colonies grown on agar plates prepared with imMedia™ Blue medium must develop a deep blue color within 16 hours of incubation at 37 C.
Answer Id: 3682
What is the shelf life of imMedia™?