Drosophila S2 Cells in Schneider′s Medium

MSDS

MSDS Files
R690
Catalog #s
R69007
35d4a87ccd61d05a1868c25ba38f8bea_MSDS

Drosophila Schneider 2 (S2) Cells.

Manual / Product Insert

Catalog #s
R69007
192fc044e74dffea144f9ac5dc9f3395_Manuals

The human cytomegalovirus immediate early gene promoter is a strong promoter in cultured Drosophila melanogaster cells.

Citations & References

  • Authors: Sinclair J H;
  • Journal: Nucleic Acids Res (1987) 15:2392-2392
  • Product Usage: CMV promoter works in Drosophila S2 cells.
  • ID: PN62824
Catalog #s
R69007
cfef40996e0de2c0f3fe1031421403fd_Citations

Drosophila Ack targets its substrate, the sorting nexin DSH3PX1, to a protein complex involved in axonal guidance.

Citations & References

  • Authors: Worby Carolyn A; Simonson-Leff Nancy; Clemens James C; Huddler Donald Jr; Muda Marco; Dixon Jack E;
  • Journal: J Biol Chem (2002) 277:9422-9430
  • Product Usage: S2 cells were transfected with the Drosophila expression vector pMT/V5-His containing wild type or mutant DAck-coding sequences for the generation of stable transformants. To induce protein synthesis, CuSO4 was added to the pMT/V5-His stable transformants at a final concentration of 500 µM 48 h prior to harvest.
  • ID: PN62239
Catalog #s
11720034
K412001
R69007
e3a413605c34e75eb80b4209a23485e3_Citations

Inhibitory Potency and Specificity of Subtilase-like Pro-protein Convertase (SPC) Prodomains.

Citations & References

  • Authors: Fugere Martin; Limperis Polizois C; Beaulieu-Audy Veronique; Gagnon Frederic; Lavigne Pierre; Klarskov Klaus; Leduc Richard; Day Robert;
  • Journal: J Biol Chem (2002) 277:7648-7704
  • Product Usage: Drosophila Schneider 2 cells (S2 cells) were grown in complete DES expression medium. Stable cell lines were established by co-transfecting the enzyme cDNA/pAC5.1/V5 HisA plasmid with pCoHYGRO plasmid at a weight ratio of 19:1 µg. PCR fragments were subcloned into the pTrcHis A vector and expressed in Ecoli.
  • ID: PN62498
Catalog #s
430025
K411001
R69007
7720dd1f89f149b7610d2607b7e7ee1c_Citations

A heterotrimeric death domain complex in Toll signaling.

Citations & References

  • Authors: Sun Huaiyu; Bristow Benjamin N; Qu Guowei; Wasserman Steven A;
  • Journal: Proc Natl Acad Sci U S A (2002) 99:12871-12877
  • Product Usage: S2 cells were transfected with 0.4 µg of appropriate reporter plasmid plus 0.4 µg of pAc-LacZ (Drosophila Expression System, Invitrogen) in a 10-cm dish 24 h before RNAi. To treat cells with dsRNA, S2 cells were washed once with complete Schneider's medium and then resuspended in serum-free medium to a density of 106 cells per milliliter
  • ID: PN65012
Catalog #s
K411001
R69007
e51ba02c4a70ef8a1a52a987473f3dee_Citations