Which serum-free media can be used to culture human keratinocytes?

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There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (catalog # 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (catalog #10744-019) or Epilife® medium (catalog # M-EPI-500-CA) supplemented with EDGS (catalog # S-012-5). If you would like an animal origin free defined system, Epilife® medium (catalog # M-EPI-500-CA) with S7 supplement (catalog # S-017-5) is an excellent choice.

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Which serum-free media can be used to culture mouse keratinocytes?

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The Keratinocyte SFM without calcium chloride (catalog # 37010-022) is appropriate for the culture of mouse keratinocytes. The medium should be supplemented with calcium chloride at a final concentration of 0.02 mM.

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Are the Keratinocyte SFM basal medium and supplement kit available separately?

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The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311.

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