The nomenclature for your Vivid Colors™ fluorescent protein-tagged vectors seems to be different from Clontech's nomenclature. Why is this?
Clontech named their BD Living Colors-fusion vectors with reference to the location of the ORF in the fusion construct. For example, to fuse an ORF with a C-terminal fluorescent protein tag, a Clontech N-series vector (N-terminal Fusion vector) was used. Our nomenclature is exactly the opposite. This was done to be consistent with the standardized nomenclature we have been using with our expression vector products for many years. For example, our Vivid Colors™ pcDNA™6.2/C-EmGFP-DEST is a vector suitable for Gateway® cloning an ORF upstream of an EmGFP-tag. Likewise, the Vivid Colors™ pcDNA™6.2/N-YFP-DEST is a vector suitable for Gateway® cloning an ORF downstream of a YFP-tag. So, our Vivid Colors™-fusion vectors are named with specific reference to the location of the fluorescent protein tag in the fusion construct.
Answer Id: 4594
How do your™ Vivid Colors™ fluorescent protein vectors compare to the previously sold Clontech BD Living Colors fluorescent protein vectors in terms of overall brightness?
In addition to the key mutations that enhanced the brightness of the Clontech fluorescent proteins, we have added further genetic enhancements to our fluorescent proteins to increase the quantum yield. Side-by-side comparisons have shown the fluorescence intensity of the our Vivid Colors™ fluorescent protein expression vectors to be at least equivalent to (or better than) the comparable Clontech BD Living Colors™ fluorescent protein expression vectors.
Answer Id: 4622