Are there potential caveats when using long primers?

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No. Longer primers work fine. Generally, 50 nmol of desalted-purity oligos are adequate for most applications. Oligos should be adjusted to a concentration of 20-50 μM and the concentration should be verified by spectrophotometry. For cloning large PCR products (>5 kb), colony output can be increased if long primers (>65 bases) are further purified (i.e., cartridge purification or PAGE). Go to www.lifetechnologies.com/customoligos for more information.

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