Manual: Applied Biosystems 3730/3730xl DNA Analyzers (English )

Manual / Product Insert

Version: 21 Jul 2010
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How can a solid yellow light on the Applied Biosystems® 3730 Series DNA Analyzer be resolved?

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Answer

If a solid yellow light remains on the Applied Biosystems® 3730 Series DNA Analyzer, perform the following steps:

1) Cycle power on the system in the proper order:
     a. Boot/Reboot the computer workstation and log in to the computer workstation
     b. Ensure that the buffer, water, and waste reservoirs are in their appropriate stations.
     c. Close the oven door, stacker drawer, and instrument doors.
     d. Cycle power on the Applied Biosystems® 3730 Series DNA Analyzer.

2) Confirm that the original communication cable is attached properly ("computer" labeled end attached to computer and "instrument" labeled end attached to instrument). NOTE: the original communication cable must be used as it is of a non-standard design.

3) Reset the "3730User" account password. To do so:
     a. Right click on "My Computer" and click on "Manage".
     b. In the Computer Management window, click on "Local Users and Groups" and click on "User".
     c. On the right side of the window, there should be a username "3730User" Right click on that and select "Set Password".
     d. Set the password to "3730User" (case sensitive). Confirm the password entry then click on "Ok".
     e. In the Computer Management window, right click on "3730User" and select "Properties". "User cannot change password" and "Password never expires" should be selected.
     f. Click on "Ok" and exit out of Computer Management window.

4) Confirm whether or not your IT department has:
     a. Changed the name of the computer workstation.
     b. Installed or configured firewall/antiviral software on the computer workstation.
     c. Altered the original IP address of the computer/network card.
     d. Configured the computer for network access.
     e. Installed any updates or patches for the Operating System/Service Pack. NOTE: These changes may disrupt communication between the instrument and the computer.

5) Ensure that the "3730calib.ini" file is in the appropriate place. The file location on the Applied Biosystems® 3730 Series DNA Analyzer running Data Collection v3.0 and v.3.1: C:/AppliedBiosystems/Firmware.

If the problems persist, contact Technical Support or Instrument Service.

Answer Id: 2546

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What polymer is supported on the Applied Biosystems® 3730 Series DNA Analyzer?

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Answer

Applied Biosystems® supports the use of POP-7 ™ polymer on the Applied Biosystems® 3730 Series DNA Analyzer. If the instrument is running Applied Biosystems® 3730 Data Collection Software v.3.1 or later, then you can also use POP-6™ polymer. We do not recommend mixing and matching of various instrument polymers as each is manufactured in different processes, tested as an entire system, and guaranteed to perform to a minimum specification. Substitution of reagents may compromise the integrity of the system and cannot be supported by AB service or applications support specialists. Using unsupported polymers may adversely affect data quality, consumable lifespan, and may void existing warranties.

Answer Id: 2527

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Does the Applied Biosystems® 3130 Series use the same POP-7™ Polymer as the Applied Biosystems® 3730 Series?

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Yes, Applied Biosystems® 3130 Series Systems use the same POP-7™ Polymer as Applied Biosystems® 3730 series instruments, but is sold in a convenient 7 mL volume bottle which is aligned with expected Applied Biosystems® 3130 Series throughput. We do not recommend customers purchase the Applied Biosystems® 3730 series sized POP-7™ Polymer due to the difference in throughput, limited shelf life and potential problems with pouring.

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What is the difference between the Water Wash Wizard and the Water Trap Flush on the 3730 Series?

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The Water Wash Wizard is an automated procedure to clean the block, polymer channels, and tubing of the Applied Biosystems® 3730 Series DNA Analyzer. The Water Trap Flush is a manual procedure to clean/refill water in the Water Trap of the Applied Biosystems® 3730 Series DNA Analyzer's Polymer Delivery Pump (PDP) block. Also refer to FAQ "What is the "Water Trap"?

Answer Id: 2539

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How often should the "Cleanup Processed Plates" command be run on the Applied Biosystems® 3730 Series DNA Analyzer?

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The Applied Biosystems® 3730 Series DNA Analyzer utilizes an Oracle® database to store raw data generated by the system. Additionally, sample files extracted from the database are created on the hard drive. Routine maintenance must be performed to ensure that the appropriate database and hard drive space is available for new data; otherwise, new runs cannot be started and error messages will be generated. The "Cleanup Processed Plates" command in the Database Manager pane will allow you to delete all processed run data and processed plate records from the database. Before running this utility, ensure that all runs have been extracted from the database.

This utility does not delete:
- Data files that have already been extracted
- Spectral data and Spectral plate records
- Spatial information

Life Technologies recommends that this procedure be performed every 400–600 runs. If the database gets too full, an error message will advise the database cleanup. Extracted data (typically in the E drive) will also build-up over time and should be archived off of the computer workstation. After archiving, the extracted data should be deleted.

Answer Id: 2547

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How long can Running Buffer stay on the Applied Biosystems® 3730 Series DNA Analyzer?

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The Running Buffer on the Applied Biosystems® 3730 Series DNA Analyzer has a use-life of 48 hours on the instrument. Replace the Running Buffer (in both the buffer jar and reservoirs) every 48 hours.

Answer Id: 2540

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How long can POP-7™ polymer stay on the Applied Biosystems® 3730 Series DNA Analyzer?

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POP-7™ polymer can maintain its performance for seven days on the Applied Biosystems® 3730 Series DNA Analyzer at standard lab conditions. After seven days, perform all required maintenance (including the Water Wash Wizard) and replace with fresh polymer. Do not "top off" the polymer bottle (add fresh polymer to old polymer), which can adversely affect data quality and consumable lifespan.

Answer Id: 2528

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What is the lifespan of an Applied Biosystems® 3730 Series DNA Analyzer capillary array?

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The life span of an array can vary depending on application, sample prep (clean-up), and excessive handling. Each Applied Biosystems® 3730 Series DNA Analyzer capillary array (regardless of length or number of capillaries) has a minimum specification of 300 sample injections per capillary under normal run conditions. This minimum specification assumes careful maintenance and proper usage of the system.

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Manual: Applied Biosystems 3730/3730xl DNA Analyzers: Sequencing Chemistry Guide: Rev B (English )

Manual / Product Insert

Version: 20 Jun 2007
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Protocol: User Bulletin 3730/3730xl DNA Analyzer Data Collection Software version 3.1 (English )

Manual / Product Insert

Version: 09 Mar 2010
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