How long can the diluted transfection lipid sit out at room temperature before losing transfection efficiency?

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This is cell line and reagent dependent but we have found that diluted RNAiMAX reagent is quite stable. We tested up to 2 hrs following transfections into HeLa cells with good knockdown. However, we noticed a difference if the lipid was diluted in a tissue culture plate versus a conical tube, but if the transfections occur within an hour there isn’t a problem.

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Citations & References

  • Authors: Torarinsson E; Yao Z; Wiklund ED; Bramsen JB; Hansen C; Kjems J; Tommerup N; Ruzzo WL; Gorodkin J
  • Journal: Genome Research 2007 2:242-251
  • ID: PN72219