How much library do I need as input into the Ion OneTouch™ 2 template preparation reaction?

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Answer

The input amount for template preparation on the Ion OneTouch™ 2 is library type specific. Occasionally, library input titration may be required to optimize templated ISP clonality and maximize sequencing throughput. Please see the specific guidelines for your library type in the appropriate library preparation user guide.
• Ion PGM™ System Library Preparation User Guides: http://ioncommunity.lifetechnologies.com/community/protocols-home/pgm-protocols/library
• Ion Proton™ System Library Preparation User Guides: http://ioncommunity.lifetechnologies.com/community/protocols-home/proton-protocols/library

Answer Id: 6879

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