What are the performance specifications of the TaqMan® miRNA assays?
The TaqMan® miRNA asays are guaranteed to meet the following:
Dynamic Range: > 6 logs10 with > 0.97 linearity (R2 value)
Specificity: Majority of assays have < 5% cross reactivity with closely related sequences. NTC background: Ct > 38.0
Lot-to-Lot Reproducibility: Difference between Cts < 0.6 Ct when different lots of an assay are run with the same sample and master mix from the same lot on the sample plate
Amplification Efficiency: ranging from 90-110% across 5 logs10
Please see the document TaqMan Assays QPCR Guarantee Program for more details.
Answer Id: 7074
Application Note: Optimization of Qdot® nanocrystals for flow cytometry
- Version: 13 April 2010
Manual / Product Insert