What is the difference between the ABI Prism® 7000 Sequence Detection System bulb and the Applied Biosystems® 7300, 7500 Real-Time PCR System bulb?
The Applied Biosystems® 7300, 7500 and 7500 FAST Real-Time PCR System use the same bulb. It is a halogen lamp that is 12V and 75W with part number 4345287. This is different from the ABI Prism® 7000 bulb which is a tungsten halogen lamp that is 21V and 150W with part number 4347754. You cannot interchange the ABI Prism® 7000 bulb with the 7300/7500/7500 FAST bulb.
Answer Id: 2581
If I were to upgrade my existing 7500 System standard unit to the a 7500 Fast configuration, can I still open my old data files?
Yes, older 7500 or 7500 fast data files are compatible with the current version of the SDS software. However, there is one exception: please note that the SDS v1.3 software will not allow RQ Studies to be created from RQ Plates from different instrument types, different thermal modes (i.e., fast and standard modes) or different numbers of thermal cycles.
Answer Id: 2341
What is the maximum thermal output for the Applied Biosystems® 7500 / 7500 Fast Sequence Detection System in BTU/h?
Can I run slower, standard thermal cycling experiments on a 7500 Fast Real-Time PCR System?
Yes. you can choose either standard or fast ramp on the 7500 Fast System for software versions 2x or greater, and a 9600 emulation thermal cycling mode is available for customers who wish to run assays based on adjusted ramp rate. However, you should be aware that we cannot guarantee identical performance to the same modes run on a standard 7500 system block.
Answer Id: 2343
How can I run a standard TaqMan® assay reaction on the Applied Biosystems® 7500 Fast Real-Time PCR System?
You can run an Applied Biosystems® 7500 Fast Real-Time PCR System instrument in Standard mode as long the following set-up points are followed:
1) The reaction volume is between 10 ul-30 ul
2) The 96-well plate is a MicroAmp® FAST 96 well reaction plate (P/N 4346907, 4346906 or 4366932)
3) Select the Run Mode to be “Standard 7500 “or “9600 Emulation” (2.0.x software versions only offer Standard or Fast - no 9600 emulation choice).
Answer Id: 2474
What are the thermal cycling parameters of the Fast mode for the 7500 Fast Real-Time PCR System?
For the 7500 Fast real time PCR systems, the thermal cycling parameters are defaulted to 50°C/2 min for UNG incubation, 95°C/20 sec for fast polymerase activation, 40 cycles of 95°C/3 secs for the dissociation and 60°C/30 seconds for annealing and extensions.
Answer Id: 2344
What are the ramp rates for the Applied Biosystems® 7500 and 7500 Fast Real-Time PCR Instruments?
The sample ramp rates for the 7500 instrument are as follows:
- Standard Mode: 1.6 deg C/sec up and 1.6 deg C/sec down
- 9600 emulation Mode: 0.8 deg C/sec up and 1.6 deg C/sec down. This matches the sample ramp rate achieved for the ABI PRISM® 7700 Sequence Detection System.
- Fast Mode: 3.5 deg C/sec up and 3.5 deg C/sec down.
Answer Id: 2481
What is the minimum reaction volume I should be using in the ABI Prism® 7300 and 7500 Sequence Detection System?
The minimum reaction volume supported by Applied Biosystems® is 20 ul for Real-Time PCR runs and Allelic Discrimination plate reads on the ABI Prism® 7300 and 7500 (Standard) Sequence Detection System. We have not validated the instruments with reactions volumes below 20 ul. We recommend you perform your own validation for comparable efficiency at lower reaction volume. On the Applied Biosystems® 7500 Fast Real-Time PCR System instrument, you can run a minimum reaction volume of 10 ul reactions.
Answer Id: 2587
Can I downgrade my Applied Biosystems® 7500 Fast Real-Time PCR System to the standard block?
How can I export the amplification plot or gene expression plot from the Applied Biosystems® 7300, 7500, and 7500 Fast Real-Time PCR System’s software?
With SDS 1.4 software, right-click on the graph, and choose "Export as JPEG" or "Export to Powerpoint". With SDS 2.0.x (for 7500/7500fast only), you can either click on the save icon above the graph "Save as JPEG Image" or click on the Export drop-down arrow (located on the upper toolbar) and choose "Send to Powerpoint"
Answer Id: 2477
Do I need to use MicroAmp® Optical Cover Compression Pads (Cat. No. 4312639) when using MicroAmp® Optical Adhesive Covers and MicroAmp 96-Well Optical Reaction Plate with Barcode (Cat. No. 4314320) on the Applied Biosystems® 7500 Real-Time PCR System?
What are the extensions of files generated by the ABI PRISM® 7000 Sequence Detection System, Applied Biosystems® 7300, 7500, 7500Fast, 7900HT Fast Real-Time PCR System, StepOnePlus™ Real-Time PCR System, and ViiA™ 7 Real-Time PCR Systems?
All run files have an .SDS or .EDS extension. A Relative Quantification study will have an .SDM or .EDM extension and a template will have an .SDT or .EDT extension. The software for StepOnePlus™, ViiA™ 7, and 7500 2.0.x software have .EDS, .EDT, or .EDM extensions; all other software have .SDS, .SDT, or .SDM extensions.
Answer Id: 2473
Manual: Applied Biosystems 7300/7500 Real-Time PCR System Getting Started Guide Installation and Maintenance(Japanese)
Manual / Product Insert
Manual: Relative Quantification: Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System: Getting Started Guide (English )
Manual / Product Insert