What is the difference between the ABI Prism® 7000 Sequence Detection System bulb and the Applied Biosystems® 7300, 7500 Real-Time PCR System bulb?

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Answer

The Applied Biosystems® 7300, 7500 and 7500 FAST Real-Time PCR System use the same bulb. It is a halogen lamp that is 12V and 75W with part number 4345287. This is different from the ABI Prism® 7000 bulb which is a tungsten halogen lamp that is 21V and 150W with part number 4347754. You cannot interchange the ABI Prism® 7000 bulb with the 7300/7500/7500 FAST bulb.

Answer Id: 2581

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309a8e73b2cdb95fc1affa8845504e87_FAQ

If I were to upgrade my existing 7500 System standard unit to the a 7500 Fast configuration, can I still open my old data files?

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Answer

Yes, older 7500 or 7500 fast data files are compatible with the current version of the SDS software. However, there is one exception: please note that the SDS v1.3 software will not allow RQ Studies to be created from RQ Plates from different instrument types, different thermal modes (i.e., fast and standard modes) or different numbers of thermal cycles.

Answer Id: 2341

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c8dfece5cc68249206e4690fc4737a8d_FAQ

Can I run slower, standard thermal cycling experiments on a 7500 Fast Real-Time PCR System?

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Answer

Yes. you can choose either standard or fast ramp on the 7500 Fast System for software versions 2x or greater, and a 9600 emulation thermal cycling mode is available for customers who wish to run assays based on adjusted ramp rate. However, you should be aware that we cannot guarantee identical performance to the same modes run on a standard 7500 system block.

Answer Id: 2343

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7cc532d783a7461f227a5da8ea80bfe1_FAQ

What is the maximum thermal output for the Applied Biosystems® 7500 / 7500 Fast Sequence Detection System in BTU/h?

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The maximum thermal output for the Applied Biosystems® 7500/7500 Fast Sequence Detection System is 3241.5 BTU/h (950W).

Answer Id: 2503

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e71e5cd119bbc5797164fb0cd7fd94a4_FAQ

How can I run a standard TaqMan® assay reaction on the Applied Biosystems® 7500 Fast Real-Time PCR System?

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Answer

You can run an Applied Biosystems® 7500 Fast Real-Time PCR System instrument in Standard mode as long the following set-up points are followed:

1) The reaction volume is between 10 ul-30 ul
2) The 96-well plate is a MicroAmp® FAST 96 well reaction plate (P/N 4346907, 4346906 or 4366932)
3) Select the Run Mode to be “Standard 7500 “or “9600 Emulation” (2.0.x software versions only offer Standard or Fast - no 9600 emulation choice).

Answer Id: 2474

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0bf727e907c5fc9d5356f11e4c45d613_FAQ

What are the thermal cycling parameters of the Fast mode for the 7500 Fast Real-Time PCR System?

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Answer

For the 7500 Fast real time PCR systems, the thermal cycling parameters are defaulted to 50°C/2 min for UNG incubation, 95°C/20 sec for fast polymerase activation, 40 cycles of 95°C/3 secs for the dissociation and 60°C/30 seconds for annealing and extensions.

Answer Id: 2344

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f52854cc99ae1c1966b0a21d0127975b_FAQ

What are the ramp rates for the Applied Biosystems® 7500 and 7500 Fast Real-Time PCR Instruments?

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Answer

The sample ramp rates for the 7500 instrument are as follows:

- Standard Mode: 1.6 deg C/sec up and 1.6 deg C/sec down
- 9600 emulation Mode: 0.8 deg C/sec up and 1.6 deg C/sec down. This matches the sample ramp rate achieved for the ABI PRISM® 7700 Sequence Detection System.
- Fast Mode: 3.5 deg C/sec up and 3.5 deg C/sec down.

Answer Id: 2481

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6b5754d737784b51ec5075c0dc437bf0_FAQ

What is the minimum reaction volume I should be using in the ABI Prism® 7300 and 7500 Sequence Detection System?

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Answer

The minimum reaction volume supported by Applied Biosystems® is 20 ul for Real-Time PCR runs and Allelic Discrimination plate reads on the ABI Prism® 7300 and 7500 (Standard) Sequence Detection System. We have not validated the instruments with reactions volumes below 20 ul. We recommend you perform your own validation for comparable efficiency at lower reaction volume. On the Applied Biosystems® 7500 Fast Real-Time PCR System instrument, you can run a minimum reaction volume of 10 ul reactions.

Answer Id: 2587

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ef0917ea498b1665ad6c701057155abe_FAQ

Can I downgrade my Applied Biosystems® 7500 Fast Real-Time PCR System to the standard block?

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No, we do not offer the option to downgrade an Applied Biosystems® Fast 7500 System to a standard block.

Answer Id: 2315

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88bfcf02e7f554f9e9ea350b699bc6a7_FAQ

Do I need to use MicroAmp® Optical Cover Compression Pads (Cat. No. 4312639) when using MicroAmp® Optical Adhesive Covers and MicroAmp 96-Well Optical Reaction Plate with Barcode (Cat. No. 4314320) on the Applied Biosystems® 7500 Real-Time PCR System?

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Answer

No, MicroAmp® Optical Cover Compression Pads should not be used on the Applied Biosystems® 7500 Real-Time PCR System.

Answer Id: 2308

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e58aea67b01fa747687f038dfde066f6_FAQ

How can I export the amplification plot or gene expression plot from the Applied Biosystems® 7300, 7500, and 7500 Fast Real-Time PCR System’s software?

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Answer

With SDS 1.4 software, right-click on the graph, and choose "Export as JPEG" or "Export to Powerpoint". With SDS 2.0.x (for 7500/7500fast only), you can either click on the save icon above the graph "Save as JPEG Image" or click on the Export drop-down arrow (located on the upper toolbar) and choose "Send to Powerpoint"

Answer Id: 2477

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4c8c76b39d294759a9000cbda3a6571a_FAQ

What are the extensions of files generated by the ABI PRISM® 7000 Sequence Detection System, Applied Biosystems® 7300, 7500, 7500Fast, 7900HT Fast Real-Time PCR System, StepOnePlus™ Real-Time PCR System, and ViiA™ 7 Real-Time PCR Systems?

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Answer

All run files have an .SDS or .EDS extension. A Relative Quantification study will have an .SDM or .EDM extension and a template will have an .SDT or .EDT extension. The software for StepOnePlus™, ViiA™ 7, and 7500 2.0.x software have .EDS, .EDT, or .EDM extensions; all other software have .SDS, .SDT, or .SDM extensions.

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Getting Started Guide Installation and Maintenance: 7300/7500 Real-Time PCR System (Japanese)

Manual / Product Insert

Catalog #s
4351101
4351103
4351104
4351105
4351106
4351107
188e30ebbc769aeda748b09cec89f0bb_Manuals

Manual: Relative Quantification: Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System: Getting Started Guide (English )

Manual / Product Insert

Catalog #s
4370199
4370200
4370202
8bb6fe84b8bba85343f415936f15e878_Manuals

SITE PREP GUIDE: 7300/7500/7500 FAST REAL TIME PCR SYSTEM

Manual / Product Insert

  • Download
  • Version: 4378654 Rev. A 9/2006
Catalog #s
4370199
4370200
4370202
ff66405dab3d52bb64fcda44b47921dd_Manuals