Gibco® B-27® Supplements
For nearly 20 years, researchers have been publishing their experiments using B-27® supplements. The first commercially available, serum-free supplement for neural cell culture, B-27® Serum Free Supplement was developed for the long-term culture of rat hippocampal and cortical neurons. Since its introduction, the publication history of B-27® supplements demonstrates that researchers are expanding the use of these supplements far beyond these original applications.
Read how a University of Wisconsin-Madison team used B-27® Minus Insulin in their cardiomyocyte differentiation study.
The most referenced neural cell culture supplement
Available exclusively from Life Technologies, the B-27® family of supplements are the most referenced neural cell culture supplements. B-27® supplements have been cited in thousands of peer-reviewed publications spanning a variety of different cell types, and used in the research of many different neurological disease models.
Which B-27® supplement is right for you?
To order the right B-27® supplement for you, click on the application/cell-type tab below and then click on the product name or catalog number to order.
All Gibco® neural cell culture reagents are optimized to work together as a complete media system. Need a complementary media or cells? Browse our neural cell culture product guide.
|Application||Product name||Catalog No.|
|Studies where XenoFree components are required||B-27® Supplement, XenoFree CTS™||A14867-01|
|Studies of oxidative stress/damage, apoptosis,
or where free radical damage to neurons occurs
minus AO (antioxidants)
|Studies of insulin secretion or insulin receptors||B-27® Supplement,
|Electrophysiology studies||B-27® Electrophysiology Kit||A1413701|
|Growth and expansion of retinal ganglion cells||B-27® Supplement, Custom||0080085SA|
Table 1. Cell types and disease models cited in publications using Gibco® neural cell culture reagents
Brainstem, cerebellar, cortical, dopaminergic, DRG, enteric, GABAergic, glutamatergic, hippocampal, motor, neural progenitor, pancreatic, parasympathetic, retinal, septal, striatal, substantia nigral, sympathetic ganglia
|Induced pluripotent stem cell, neural precursor, neural stem cell, neuroepithelial, cardiomyocytes|
|Astrocyte, microglia, oligodendrocytes, radial glia, satellite, schwann|
|Disease Models||Alzheimer’s disease, amyotropyic lateral sclerosis (ALS), brain tumor, cerebral palsy, epilepsy, glioma, Huntington’s disease, multiple sclerosis, Parkinson’s disease, schizophrenia, spinal cord injury, stroke|
Neural Cell Culture Resources
For research use only. Not for human or animal therapeutic or diagnostic use.