Find the KnockOut™ products available for different culture condition requirements.

Having the right components for your complete cell culture medium is essential to achieving the best cell culture results. KnockOut™ media and reagents provide the optimal culture conditions for mouse and human pluripotent stem cells (PSC).

To order the optimal KnockOut™ media system for your culture conditions, click on the appropriate tab below.

To prepare most complete media, a basal medium and a supplement will be required. Additional considerations for each condition type are noted above each ordering table.

Feeder-dependent

Culturing PSCs on a layer of feeder cells in KnockOut™ Serum Replacement supplemented medium

A feeder layer, traditionally comprised of inactivated mouse embryonic fibroblasts (MEF), serves as an adherent surface for the PSCs. While the feeder layer does not replicate in the culture dish, it plays a supporting role in supplying required nutrients and matrix proteins for the PSCs.

Basal Media Supplements Cells Cost-effective Media System
View instructions to prepare complete medium
 

For research use only. Not for human or animal therapeutic or diagnostic use.

Conditioned Medium

A KnockOut™ Serum Replacement medium that supports the growth of PSCs in the absence of a feeder layer.

Conditioned medium is KnockOut™ Serum Replacement supplemented medium that has been conditioned on a layer of feeder cells, and therefore can be used to grow PSCs in the absence of a feeder layer. In the absence of a feeder layer, an extracellular matrix is required, such as Geltrex® LDEV-Free hESC-qualified Matrix, in order for the PSCs to adhere to the growing surface.

Basal Media Supplements Matrix Cost-effective
Media System

Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium

Culturing Induced Pluripotent Stem Cells (iPSCs) on MEF-Conditioned Medium

For research use only. Not for human or animal therapeutic or diagnostic use.

Feeder-free

Nutrient-rich media that does not require a feeder-layer of cells

Feeder free media are nutrient-rich and do not require a feeder-layer of cells. KnockOut™ Serum Replacement supplemented medium is not recommended for growing PSCs feeder-free, however we do recommend the use of StemPro® hESC SFM.

In a feeder-free system, an extracellular matrix is required, such as Geltrex® LDEV-Free hESC-qualified Matrix.

Feeder-based, xeno-free medium:

Culturing PSCs in xeno-free conditions with feeders requires the use of human feeders, typically human foreskin fibroblasts.

The contents of the KnockOut™ SR XenoFree CTS™ Kit includes a bottle of KnockOut™ SR XenoFree CTS™ and a product manual.

Media Matrix
 

Feeder-free, xeno-free medium:

Growing PSCs in xeno-free conditions in the absence of feeders requires additional growth factors to maintain pluripotency. To prepare complete medium, chose a basal medium, a supplement, and the growth factor supplement.   In the absence of a feeder layer, a humanized extracellular matrix is required, such as CELLstart™ CTS™, in order for the PSCs to adhere to the growing surface.

Basal Media Supplements Growth Factor Supplement Matrix
View instructions to prepare complete medium
  

For research use only. Not for human or animal therapeutic or diagnostic use.

Xeno-free

PSC culture media comprised of only human-origin or recombinant components

The removal of animal-derived components from PSC culture media provides a xeno-free medium which can facilitate the transition of PSC research to clinical research applications. The Gibco® Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices.

Feeder-based, xeno-free medium:

Culturing PSCs in xeno-free conditions with feeders requires the use of human feeders, typically human foreskin fibroblasts.

The contents of the KnockOut™ SR XenoFree CTS™ Kit includes a bottle of KnockOut™ SR XenoFree CTS™ and a product manual.

Basal Media Supplements Cost-effective Media System
View instructions to prepare complete medium

Feeder-free, xeno-free medium:

Growing PSCs in xeno-free conditions in the absence of feeders requires additional growth factors to maintain pluripotency. To prepare complete medium, chose a basal medium, a supplement, and the growth factor supplement.   In the absence of a feeder layer, a humanized extracellular matrix is required, such as CELLstart™ CTS™, in order for the PSCs to adhere to the growing surface.

Basal Media Supplements Growth Factor Supplement Matrix
View instructions to prepare complete medium
  

For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal Law restricts this device to sale by or on the order of a physician.