CyQUANT® Cell Proliferation AssaysThe amount of DNA in each cell remains constant for a given cell line or cell type, so assays based on DNA content—like CyQUANT® Cell Proliferation Assays—can be used to provide an accurate and simple measure of cell number. CyQUANT® Cell Proliferation Assays are ideal for high-throughput screening, are more sensitive than colorimetric-based assays, and are not radioactive. Rapid and easy to use, CyQUANT® Cell Proliferation Assays Kits require no washes, extractions, growth medium changes, or long incubations, and don't rely metabolic status of the cell.

CyQUANT® Cell Proliferation Assays Kits selection guide

 
Linearity (# of cells)
50–50,000
100–20,000
50–20,000
Length of assay
3 hrs minimum
1 hr
1 hr
Optimal assay design
Multi-day or endpoint experiment
Real-time data analysis
High-throughput in 96-, 384-, or 1,536-well plates
Freezing step required?
Yes
No
No
Media removal required?
Yes
Yes
No
Sample volume
200 µL
100 µL
5–100 µL (reagent is added directly to culture medium in microwells)
Ex/Em
480/520 nm
497/520 nm
508/527 nm
Validated cell lines Mouse fibroblasts (NIH 3T3 and CRE­BAG 2 cells), normal human umbilical vein endothelial cells (HUVEC), canine kidney cells (MDCK), chinook salmon embryo cells (CHSE), rat basophilic leukemia cells (RBL), rat glioma cells (C6), and mouse myeloma cells (P3X63A68) HEK 293, HeLa, HepG2, 3T3 and CHO cells Adherent (CHO-K1, Hep-G2, U87, MDA-MB-231, SNU-1, PC-3, human primary aortic smooth muscle cells (HASMC), human primary pulmonary arterial smooth muscle cells (HPASMC), human keratinocytes, and HUVEC) and suspension cells (Jurkat)

CyQUANT® Cell Proliferation Assays

  • For multi-day tests or when you need precision
  • Samples are run, frozen, then assayed all at the same time using the same set of standards
  • Freezing helps ensure precision
  • Linear from 50 to 50,000 cells (Figure 1)
  • Up to 250,000 cells with increased dye concentration

CyQUANT® Cell Proliferation Assays

CyQUANT® Cell Proliferation Assay  
Figure 1. Quantitation of NIH 3T3 fibroblasts using the CyQUANT® Cell Proliferation Assay Kit. Fluorescence measurements were made using a microplate reader with excitation at 485 nm and emission detection at 530 nm. The linear range of the assay under these conditions is from 50 to 50,000 cells per 200 µL sample. The inset shows the linearity that can be obtained at very low number of cells.

CyQUANT® NF Cell Proliferation Assays

  • When you need real-time data
  • Streamlined–1 hour start to finish
  • No freeze step required
  • Linear from 100 to 20,000 cells (Figure 2)

CyQUANT® NF Cell Proliferation Assays

CyQUANT® NF Cell Proliferation Assay  

Figure 2. Quantitation of CHO (M1WT3) cells using the CyQUANT® NF Cell Proliferation Assay Kit. Fluorescence measurements were made using a microplate reader with excitation at 485 nm and emission detection at 530 nm. The linear range of the assay under these conditions is from 100 to 20,000 cells per 100 µL sample. The inset shows the linearity that can be obtained at very low number of cells.