Innovative science requires versatile tools. Invitrogen provides researchers with just such a tool—Qdot® nanocrystals with varied surface chemistries. These products provide the highest degree of flexibility in nanocrystal-based experimental design, offering:
Qdot® Innovator's Tool Kit ITK™ nanocrystals enable researchers to achieve custom Qdot® nanocrystal labeling of nearly any material of interest. Qdot® ITK™ nanocrystals are available with three different surface chemistries—carboxyl groups, amino groups, or organic-soluble groups—for extremely versatile labeling options. These materials provide a remarkable platform for the development of nanocrystal-based assays; for example, they allow researchers to modify Qdot® nanocrystals in a controlled stoichiometric fashion, and to experiment with any number of functional surface modifications. The customizable surface of Qdot® ITK™ nanocrystals should prove particularly useful in the preparation of probes with multiple surface functionalities for powerful, data-rich assays.
Qdot® ITK™ amino (PEG) nanocrystals can be coupled by a wide variety of standard amine-reactive crosslinking chemistries (top). Qdot® ITK™ carboxyl nanocrystals can be coupled using standard EDC (carbodiimide) activation and coupling chemistries (bottom).
Qdot® products combine the revolutionary fluorescence performance inherent in the nanocrystal structure with a highly customizable surface for directing the bioactivity of Qdot® nanocrystals or for conjugating them to a wide range of molecules of interest. Qdot® nanocrystals represent a truly enabling "nanotechnology"—the many benefits they bring to fluorescence methodology include:
Long-term photostability for live-cell imaging and dynamics studies
Fixability for follow-up immunofluorescence from in vivo studies
Archivability for permanent sample storage in pathology
Brilliant colors for simple, single-excitation source, multicolor analysis