ReadyProbes® Ready-to-Use Imaging Reagents  

The easiest way to image your cells

ReadyProbes® reagents are ready-to-use imaging solutions designed to allow you to stain cells with:

  • No calculations
  • No dilutions
  • No pipetting

 

Reach for the convenient dropper bottle, add 2 drops per milliliter of your cell suspension, and you're ready to image. ReadyProbes® reagents address your most common imaging needs, including nuclear and cytoskeletal staining, and analyses for cell viability and apoptosis. In addition, we’ve formulated some of our most popular secondary antibodies for use straight from the dropper bottle. We’ve also developed ReadyProbes® imaging accessories to help you get the best results from your staining protocol. Imaging has never been easier.


ReadyProbes® Cell Stains

With each ReadyProbes® reagent, you can follow the icon-based protocol to obtain quick and easy results for a common imaging application. ReadyProbes® cell stains can remain on the bench within arm’s reach of your microscope. Add 2 drops per milliliter of solution, and you're ready for the next step in your experiment.

 
NucBlue® Live ReadyProbes® Reagent
NucRed® Live 647 ReadyProbes® Reagent
NucBlue® Fixed Cell ReadyProbes® Reagent
Readout 
The easiest fluorescent staining method for nucleic acids;  no calculations, no dilutions, no pipetting
Target
High-efficiency staining of both RNA and DNA
Common filter set 
DAPI
Cy®5
DAPI
Ex/Em (nm) 
358/461
638/686
360/460
Signal-to-noise ratio
Photostability
Live cells
Yes
Yes
No
Fixed cells
Yes
Yes
Yes
Fixable
Yes
Yes
NA
Format 
6 x dropper bottles
6 x dropper bottles
6 x dropper bottles
Cat. No.
R37605
R37106
R37606

See more nuclear staining reagents

 
ActinGreen™ 488 ReadyProbes® Reagent
ActinRed™ 555 ReadyProbes® Reagent
Readout 
The easiest fluorescent staining method for actin;  no calculations, no dilutions, no pipetting
Target
High-affinity staining of F-actin
Common filter set 
FITC
TRITC
Ex/Em (nm) 
495/518
555/565
Signal-to-noise ratio
 
Photostability
 
 
Live cells
No
No
Fixed cells
Yes
Yes
Fixable
NA
NA
Platforms
Imaging
Imaging
Format 
6 x dropper bottles
6 x dropper bottles
Cat. No.
R37110
R37112

See more actin staining reagents

NucGreen® Dead 488 ReadyProbes® Reagent
NucRed® Dead 647 ReadyProbes® Reagent
ReadyProbes® Cell Viability Imaging Kit (Blue/Green)
ReadyProbes® Cell Viability Imaging Kit (Blue/Red)
Readout 
The easiest fluorescent staining method for viability; no calculations, no dilutions, no pipetting
Target
Single-color assays detect dead cells
Two-color assays detect dead cells and total cells
Dead cells stain green
Dead cells stain red
Dead cells stain green, live cells appear blue
Dead cells stain red, total cells appear blue
Fluorescent label
DNA stain
DNA stain
DNA stain
Propidium iodide
Hoechst 33342
Hoechst 33342
Standard filter set
FITC
Cy®5
FITC
TRITC/RFP
DAPI
DAPI
Ex/Em (nm)
504/523
642/661
504/523
535/617
360/460
360/460
Platforms
Imaging
Imaging
Imaging
Imaging
Format 
6 x dropper bottles
6 x dropper bottles
6 x dropper bottles
6 x dropper bottles
Cat. No.
R37109
R37113
R37609
R37610

See more cell viability stains

 
CellEvent® Caspase-3/7 Green ReadyProbes® Reagent
Readout 
The easiest fluorescent staining method for apoptosis; no calculations, no dilutions, no pipetting
Target
Fluorescence intensity increases with caspase 3/7 activity
Common filter set 
FITC 
Ex/Em (nm) 
502/530
Live cells
Yes
Fixed cells
No
Fixable
Yes
Platforms
Imaging
Format 
6 x dropper bottles
Cat. No.
R37111

See more apoptosis stains

ReadyProbes® Secondary Antibodies

ReadyProbes® secondary antibodies are conjugated with bright and photostable Alexa Fluor® 488 and Alexa Fluor® 594 dyes to offer convenient and robust labeling. These Alexa Fluor® secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from either mouse or rabbit. Like other ReadyProbes® reagents, these secondary antibodies are provided in a convenient dropper bottle format. No pipetting or dilutions are required. Just add 2 drops per milliliter of buffer to stain your cells, and follow the icon-based protocol.

Detection with Alexa Fluor® 488 Goat Anti–Rabbit IgG Antibody ReadyProbes® Reagent
CAKI cell peroxisomes labeled using a rabbit anti-PMP70 antibody, followed by detection with Alexa Fluor® 488 Goat Anti–Rabbit IgG Antibody ReadyProbes® Reagent.

 
Alexa Fluor® 488
Alexa Fluor® 594
Common filter
FITC
Texas Red®
Ex/Em (nm)
495/518
590/617
Conjugate
Cat. No.
      Donkey anti-mouse IgG antibody
      Goat anti-rabbit IgG antibody
      Donkey anti-rabbit IgG antibody
      Goat anti-mouse IgG antibody

See more secondary antibodies


ReadyProbes® Imaging Accessories

ReadyProbes® imaging accessories are reagents that make sample staining and image analysis much easier. Whether you are new to fluorescence imaging or an experienced user trying to streamline your protocols, ReadyProbes® reagents allow you to spend less time preparing solutions and more time analyzing results. These ready-to-use imaging reagents are optimized to fix and permeabilize your cells or reduce nonspecific background signal, so you can move on quickly to the next step.

HeLa cells fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit
HeLa cells fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit.

For Research Use Only. Not for use in diagnostic procedures.