Invitrogen offers Molecular Probes® cell-impermeant nucleic acid  SYTOX® Dead Cell Stains.  Because they do not cross intact cell membranes  and also increase fluorescence upon dsDNA binding they are among our most brilliant dead cell stains.  Easy to use, SYTOX® dead cell stains can be applied to cells and visualized without an additional wash step because they are non-fluorescent in aqueous media. These stains can be used on multiple platforms including fluorescence microscopy, flow cytometry, and microplates.

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SYTOX® Green Stains

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SYTOX® Green dead cell stain has the highest nucleic acid affinity of all of the SYTOX® dead cell stains. Exhibiting a 100-fold increase in fluorescence upon nucleic acid binding and minimal base selectivity (unlike DAPI and propidium iodide), SYTOX® Green dead cell stain is great for multi-parametric analysis with blue- or red- fluorescent labels.

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SYTOX® Orange Nucleic Acid Stain

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SYTOX® Orange dead cell stain binds nucleic acids in cells with compromised membranes. This stain is useful as an indicator of cell death and is much brighter than propidium iodide.

SYTOX® Blue Stains

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SYTOX® Blue dead cell stain is a simple and quantitative single-step dead-cell indicator for use with violet laser equipped flow cytometers. SYTOX® Blue dead cell stain is a high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranes but will not cross uncompromised cell membranes.

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SYTOX® Red Dead Cell Stain

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SYTOX® Red dead cell stain is a simple and quantitative single-step dead-cell indicator for use with red laser-equipped flow cytometers. SYTOX® Red dead cell stain is a high-affinity nucleic acid stain that easily penetrates cells with com- promised plasma membranes but will not cross uncompromised cell membranes.

SYTOX® Dead Stain Cells in Action

 
Nuclear deformation of an apoptotic cell visualized with the SYTOX® Green nucleic acid stain.
 
Induced Jurkat cells stained with SYTOX® Green nucleic acid stain analyzed by flow cytometry. 
   
 
Micrococcus luteus stained with SYTOX® Blue nucleic acid stain.
 
SYTOX® Blue dead cell stain using a mixture of heat-killed and untreated Jurkat cells.
   
 
HeLa cells labeled with anti–Golgin-97 antibody and detected using Alexa Fluor® 647 goat anti–mouse IgG antibody and SYTOX® Orange dead cell stain.
 
SYTOX®  Red dead cell stain using untreated and heat-treated human peripheral blood leucoytes.

Assays using SYTOX® Green Dead Cell Stain

Ordering Information

Sku Name Size Price Qty
S11368 SYTOX® Orange Nucleic Acid Stain - 5 mM Solution in DMSO 250 µL USD 212.00
S7020 SYTOX® Green Nucleic Acid Stain - 5 mM Solution in DMSO 250 µL USD 201.00
S34857 SYTOX® Blue Dead Cell Stain, for flow cytometry 1 mL USD 288.00
S34859 SYTOX® Red Dead Cell Stain, for 633 or 635 nm excitation 1 mL USD 341.00
S11348 SYTOX® Blue Nucleic Acid Stain - 5 mM Solution in DMSO 250 µL USD 206.00
S33025 SelectFX® Nuclear Labeling Kit (DAPI, SYTOX® Green, 7-AAD, TO-PRO®-3 Iodide), for fixed cells 1 kit USD 137.00
V35113 Dead Cell Apoptosis Kit with Annexin V APC and SYTOX® Green, for flow cytometry 1 kit USD 473.00
V35112 Dead Cell Apoptosis Kit with Annexin V PE and SYTOX® Green, for flow cytometry 1 kit USD 494.00
V13240 Single-Channel Dead Cell Apoptosis Kit with Annexin V Alexa Fluor® 488 and SYTOX® Green Dyes, for flow cytometry 1 kit USD 315.00
L34951 LIVE/DEAD® Cell Vitality Assay Kit, C12 Resazurin/SYTOX® Green 1 kit USD 295.00
V35114 Metabolic Activity Dead Cell Apoptosis Kit with C12 Resazurin, Annexin V APC, and SYTOX® Green, for flow cytometry 1 kit USD 636.00
F24631 FluoCells® Prepared Slide #4 (mouse intestine section with Alexa Fluor® 350 WGA, Alexa Fluor® 568 Phalloidin, and SYTOX® Green) 1 each USD 191.00
S34860 SYTOX® Green Dead Cell Stain, for flow cytometry 1 mL USD 254.00
S34861 SYTOX® Orange Dead Cell Stain, for flow cytometry 1 mL USD 254.00
S34862 SYTOX® Dead Cell Stain Sampler Kit, for flow cytometry 1 kit USD 95.75