Our Molecular Probes® cell-impermeant nucleic acid SYTOX® Dead Cell Stains do not cross intact cell membranes and exhibit increased fluorescence upon dsDNA binding, making them some of our most brilliant dead cell stains. Easy to use, SYTOX® dead cell stains can be applied to cells and visualized without an additional wash step because they are non-fluorescent in aqueous media. These stains can be used on multiple platforms including fluorescence microscopy, flow cytometry, and microplates.
View all SYTOX® Dead Cell Stain products
SYTOX® Blue stains
SYTOX® Blue dead cell stain is a simple and quantitative single-step dead-cell indicator for use with violet laser–equipped flow cytometers. SYTOX® Blue dead cell stain is a high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranes but will not cross uncompromised cell membranes.
Micrococcus luteus stained with SYTOX® Blue nucleic acid stain.
|SYTOX® Blue dead cell stain using a mixture of heat-killed and untreated Jurkat cells.|
SYTOX® Green stains
SYTOX® Green dead cell stain has the highest nucleic acid affinity of all of the SYTOX® dead cell stains. Exhibiting a 100-fold increase in fluorescence upon nucleic acid binding and minimal base selectivity (unlike DAPI and propidium iodide), SYTOX® Green dead cell stain is great for multi-parametric analysis with blue- or red- fluorescent labels.
|Nuclear deformation of an apoptotic cell visualized with the SYTOX® Green nucleic acid stain.|
|Induced Jurkat cells stained with SYTOX® Green nucleic acid stain analyzed by flow cytometry.|
SYTOX® Orange Nucleic Acid Stain
SYTOX® Orange dead cell stain binds nucleic acids in cells with compromised membranes. This stain is useful as an indicator of cell death and is much brighter than propidium iodide.
HeLa cells labeled with anti–Golgin-97 antibody and detected using Alexa Fluor® 647 goat anti–mouse IgG antibody and SYTOX® Orange Nucleic Acid stain.
SYTOX® Red Dead Cell Stain
SYTOX® Red dead cell stain is a simple and quantitative single-step dead-cell indicator for use with red laser-equipped flow cytometers. SYTOX® Red dead cell stain is a high-affinity nucleic acid stain that easily penetrates cells with com- promised plasma membranes but will not cross uncompromised cell membranes.
SYTOX® Red dead cell stain using untreated and heat-treated human peripheral blood leucoytes.
Spectral characteristics of SYTOX® Dead Cell Stains
|Dye name||Excitation source||Excitation max (nm)||Emission max (nm)|
|SYTOX® Blue Dead Cell Stain, for flow cytometry||UV, 405, 440||470||480|
|SYTOX® Blue Nucleic Acid Cell Stain||UV, 405, 440||470||480|
|SYTOX® Green Dead Cell Stain, for flow cytometry||488||504||523|
|SYTOX® Green Nucleic Acid Stain||488||504||523|
|SYTOX® Orange Dead Cell Stain, for flow cytometry||532||547||570|
|SYTOX® Orange Nucleic Acid Stain||532||547||570|
|SYTOX® Red Dead Cell Stain||633 or 635||640||658|
|SYTOX® AADvanced™ Dead Cell Stain||488, 532||546||647|
|SYTOX® Dead Cell Stain Sampler Kit||All||All||All|
Assays using SYTOX® Green Dead Cell Stain
We have developed a variety of assays using the versatile SYTOX® Green dead cell stain including kits for apoptosis, cell viability and metabolism, as well as for staining fixed cells for fluorescence imaging.
- Dead Cell Apoptosis Kit with Annexin V PE and SYTOX® Green, for flow cytometry
- Dead Cell Apoptosis Kit with Annexin V APC and SYTOX® Green, for flow cytometry
- Metabolic Activity Dead Cell Apoptosis Kit with C12 Resazurin, Annexin V APC, and SYTOX® Green, for flow cytometry
- Single-Channel Dead Cell Apoptosis Kit with Annexin V Alexa Fluor® 488 and SYTOX® Green Dyes, for flow cytometry