SYTOX® Dead Cell StainsOur Molecular Probes® cell-impermeant nucleic acid SYTOX® Dead Cell Stains do not cross intact cell membranes  and exhibit increased fluorescence upon dsDNA binding, making them some of our most brilliant dead cell stains. Easy to use, SYTOX® dead cell stains can be applied to cells and visualized without an additional wash step because they are non-fluorescent in aqueous media. These stains can be used on multiple platforms including fluorescence microscopy, flow cytometry, and microplates.

View all SYTOX® Dead Cell Stain products

 

SYTOX® Blue stains

SYTOX® Blue dead cell stain is a simple and quantitative single-step dead-cell indicator for use with violet laser–equipped flow cytometers. SYTOX® Blue dead cell stain is a high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranes but will not cross uncompromised cell membranes.


Micrococcus luteus stained with SYTOX® Blue nucleic acid stain.

SYTOX® Blue dead cell stain using a mixture of heat-killed and untreated Jurkat cells.

SYTOX® Green stains

SYTOX® Green dead cell stain has the highest nucleic acid affinity of all of the SYTOX® dead cell stains. Exhibiting a 100-fold increase in fluorescence upon nucleic acid binding and minimal base selectivity (unlike DAPI and propidium iodide), SYTOX® Green dead cell stain is great for multi-parametric analysis with blue- or red- fluorescent labels.

Nuclear deformation of an apoptotic cell visualized with the SYTOX® Green nucleic acid stain.
Induced Jurkat cells stained with SYTOX® Green nucleic acid stain analyzed by flow cytometry. 

SYTOX® Orange Nucleic Acid Stain

SYTOX® Orange dead cell stain binds nucleic acids in cells with compromised membranes. This stain is useful as an indicator of cell death and is much brighter than propidium iodide.

 

 

 

HeLa cells labeled with anti–Golgin-97 antibody and detected using Alexa Fluor® 647 goat anti–mouse IgG antibody and SYTOX® Orange Nucleic Acid stain.

SYTOX® Red Dead Cell Stain

SYTOX® Red dead cell stain is a simple and quantitative single-step dead-cell indicator for use with red laser-equipped flow cytometers. SYTOX® Red dead cell stain is a high-affinity nucleic acid stain that easily penetrates cells with com- promised plasma membranes but will not cross uncompromised cell membranes.

 

 

 

SYTOX®  Red dead cell stain using untreated and heat-treated human peripheral blood leucoytes.

Spectral characteristics of SYTOX® Dead Cell Stains

Dye name Excitation source Excitation max (nm) Emission max (nm)
SYTOX® Blue Dead Cell Stain, for flow cytometry UV, 405, 440 470 480
SYTOX® Blue Nucleic Acid Cell Stain UV, 405, 440 470 480
SYTOX® Green Dead Cell Stain, for flow cytometry 488 504 523
SYTOX® Green Nucleic Acid Stain 488 504 523
SYTOX® Orange Dead Cell Stain, for flow cytometry 532 547 570
SYTOX® Orange Nucleic Acid Stain 532 547 570
SYTOX® Red Dead Cell Stain 633 or 635 640 658
SYTOX® AADvanced™ Dead Cell Stain 488, 532 546 647
SYTOX® Dead Cell Stain Sampler Kit All All All

Ordering information