Countess® Automated Cell Counter

Automated cell counting that is FAST, EASY and ACCURATE.

  • Helps eliminate subjectivity, minimizes human error
  • Comprehensive, reproducible data
  • Convenient data archiving and dilution calculator. Why count manually?

The Countess Automated Cell Counter can get your data in under 30 seconds without using a hemocytometer.

Purchase your Countess® Today!


Countess® Automated Cell Counter

The Countess® automated cell counter uses state-of-the-art optics and image analysis to automate cell counting. The Countess® automated cell counter is a benchtop counter designed to measure cell count and viability (live, dead, and total cells) accurately and precisely, using the standard trypan blue technique.

Using the same amount of sample that you currently use with the hemocytometer, the Countess® automated cell counter takes 30 seconds per sample for a typical cell count and is compatible with a wide variety of eukaryotic cells and provides information on cell size. Just mix 10 μL of sample with 10 μL of trypan blue, and pipet into Countess® chamber slide. Insert the slide into the instrument, press the “Count Cells” button, and get your results displayed in 30 seconds!

A single, sample measurement in 30 seconds provides the following data:

  • Live and dead cell concentration/mL
  • Total cell concentration/mL
  • Viability (% live cells to total cells)
  • Mean diameter
  • Cell images
  • Graphical data representation

The Countess® automated cell counter offers an intuitive user interface, and provides the option to save and print cell count data using the Countess software and USB drive supplied with the instrument or available separately.

The Countess® automated cell counter is supplied with disposable Countess® cell counting chamber slides that contain two enclosed chambers to hold the sample to allow you to measure two different samples or perform replicates of the same sample. The cell counting occurs in the central location of the counting chamber and the volume counted is 0.4 μL, the same as counting four (1 mm × 1 mm) squares in a standard hemocytometer.

Product information

Product Specifications.pdf
Countess Brochure.pdf
Product Manual.pdf
Countess® Citations
Quick Reference Card
Countess IQ/OQ Brochure.pdf
Owners Manual.pdf
Installation for Updates Instructions.pdf


Countess FAQs

Countess Product FAQs.pdf
Countess Firmware Update FAQs.pdf

Instrument specifications

Processing time 30 seconds
Cell sample range 1 x 104 - 1 x 107 cells/ml
Optimal cell size 5 µm to 60 µm
Instrument type Benchtop cell counter
Software Countess software
Countess USB drive 1 Gigabyte
Instrument dimensions 27 cm (w) x 20 cm (d) x 19 cm (h)
Weight 2.1 kg (4.6 lbs.)

Countess® cell counting chamber slide specifications

Chamber volume            10 µL
Material Polymethylmethacrylate (PMMA)
Dimensions   75 mm (w) x 25 mm (d) x 1.8 mm (h)
Chamber depth   100 µm


Environmental conditions

Operating power 100-240 VAC, 1.5A
Frequency 50/60 Hz
Electrical input 12 VDC, 1.0 A
Operating temperature 10-35C
Maximum relative humidity 20-80%
Altitude < 2000 m
Transient category Installment categories 11
Pollution Degree 2


Validated stem cell lines

Cell name (click for PDF) Cell type Cell size (µm in diameter)
ADSC Human fat 16-17 µm
ESC Mouse embryonic stem cells 10 µm
HESC Human embryonic stem cells 12-13 µm
NSC Rat fetal neuronal stem cells 11 µm


Validated cell lines

Cell name (click for PDF) Cell type Cell size (µm in diameter
A431 Human skin 15.5 µm
ADSC Human fat 16-17 µm
CHO-M1 Hamster ovary na
CHSE Fish embryonic 16 - 17 µm
COLO-205 Human intestine na
COS-7 Monkey kidney na
ESC Mouse embryonic stem cells 10 µm
HASMC Human smooth muscle 20 µm
HEK-293 Human kidney 13 µm
HEKn Human epidermal keratinocytes, neonstal 12.5 µm
HepG2 Human liver 18 µm
HESC Human embryonic stem cells 12 - 13 µm
HL-60 Human blood na
HPAEC Human pulmonary artery endothelial 13 µm
HPASMC Human smooth muscle 20 µm
HUVEC Human umbilical vein 17 µm
J774(MMM) Mouse blood 13-14 µm
Jurkat Human blood 12 µm
K-562 Human bone/marrow na
MCF-7 Human mammary 20-24 µm
MRC-5 HUman lung 18 µm
NIH/3T3 Mouse embryonic 18 µm
NSC Rat fetal neuronal stem cells 11 µm
PC-12 Rat adrenal 12-14 µm
SF-21 Insect ovary na
U-2 OS Human bone/marrow na
U266 Human blood 12-13 µm
Countess® Updates Download

Before installing

1. Save any data from csv file you wish to archive
   a. Go to ‘Save’ menu
   b. Press ‘View csv file’
   c. Press ‘save to USB’
2. Start a new CSV file
   a. Go to ‘Save’ screen
   b. Press ‘Start new CSV’
   c. When prompted, press ‘OK’ to erase previous data and start new csv file

Install updates

1. Download and save .exe file to your USB drive
2. Open .exe file and click ‘Extract’ (5 files will be unzipped onto your USB)
3. Insert USB drive into Countess USB port
4. Access ‘Settings’ screen and press ‘Update’
5. Press ‘OK’ after being prompted to update
6. When update is complete, you will be prompted to restart the instrument.


1. Press ‘Settings’ menu
2. Press ‘Calibrate’ button
3. Follow instructions on the calibration screen
4. Once complete, you will be prompted to restart the instrument

Update your PC software

In order to view the new Countess features on your computer, you will need to install the latest software update.

Note: The software is not required to run the instrument. Install the software only if you'd like to see the results on your computer and print a summary report.

Download installation instructions

Countess® Automated Cell Counter

Countess® slides and accessory products