The interaction between streptavidin and biotin is formed very rapidly. Once formed, the interaction is unaffected by wide extremes of pH, temperature, organic solvents and other denaturing agents.

We have a Dynabeads Streptavidin TRIAL KIT with 1 ml of each bead-type, so you can try them out with your own application.
The table below describes some typical elution efficiencies of the biotin-streptavidin interaction under a variety of elution conditions. Please note that the elution efficiencies described are for free biotin, and might differ from that of a biotinylated specific ligand.

Temperature & Time Elution Solution Elution Efficiency
90°C for 10 minutes10 mM EDTA pH 8.2 and 95% formamide
96.8%
90°C for 5 minutes10 mM EDTA pH 8.2 and 95% formamide96.4%
90°C for 2 minutes10 mM EDTA pH 8.2 and 95% formamide96.8%
65°C for 5 minutes10 mM EDTA pH 8.2 and 95% formamide96.4%
65°C for 2 minutes10 mM EDTA pH 8.2 and 95% formamide
97.9%
37°C for 10 minutes10 mM EDTA pH 8.2 and 95% formamide41.9%
90°C for 10 minutesH2O
7.3%
90°C for 10 minutes10 mM EDTA pH 8.2
52.0%
90°C for 10 minutes95% formamide
35.9%
90°C for 10 minutes30 mM NaOAc pH 9 and 95% formamide95.5%
90°C for 10 minutes80 mM NaOAc pH 9 and 95% formamide
97.3%
90°C for 10 minutes140 mM NaOAc pH 9 and 95% formamide95.4%