Quant-iT™ assay kits utilize advanced fluorophores that become fluorescent upon binding to DNA, RNA, or proteins; the fluorescence intensity of the resulting complex depends directly on the amount of the target molecule in the sample.

  • Accuracy—Quant-iT™ technology gives more accurate results than can be obtained with UV absorbance readings, because the Quant-iT™ reagents only report the molecule of interest—there’s no interference from free nucleotides or other contaminants.
  • Sensitivity—Upon binding to DNA, RNA, or protein, the fluorescence of the Quant-iT™ dyes increases several hundred fold, giving a very high signal-to-background ratio. High signal and low background mean exceedingly high sensitivity—up to 1000 times more sensitive than absorbance readings.
  • Confidence—Confidence comes from knowing your assay is sensitive and accurate enough to quantitate even the most dilute or low-abundance samples, while still leaving enough of your biomolecule for successful downstream applications. With Quant-iT™ technology, you get the crucial quantitation data you need, without wasting your sample.

Qubit® assays for use with the Qubit® 2.0 Fluorometer

For everyday needs when quantitating 1–20 samples, use Qubit® Assays for use with the Qubit® Fluorometer. Ideal for cloning, sequencing, transfection, qPCR, microarrays, protein gels, Western blots and protein activity assays. These kits are formulated for use with the Qubit® 2.0 Fluorometer (Q32866). Also available is the Qubit™ Quantitation Starter Kit (Q32871), which contains the Qubit® 2.0 Fluorometer and four Qubit™ Assay Kits.

Quant-iT™ assay kits and reagents for use with microplate readers*—for 20–2,000 samples

Product Target Useful range † Ex/Em ‡ No. of assays Cat. No.
Quant-iT™ dsDNA High-Sensitivity (HS) Assay Kit
dsDNA 0.2–100 ng 510/527 1,000 Q33120
Quant-iT™ dsDNA Broad-Range (BR) Assay Kit
dsDNA 2–1,000 ng 510/527 1,000 Q33130
Quant-iT™ RNA Assay Kit
Quant-iT™ RNA Assay Reagent
RNA 5–100 ng 644/673 1,000 Q33140
Q32844
Quant-iT™ RNA Broad-Range BR Assay Kit
RNA 20–1,000 ng 644/63 1,000 Q10213
Quant-iT™ microRNA Assay Kit
Quant-iT™ microRNA Reagent
microRNA 1–500 ng 498/518 1,000 Q32882
Q32883
Quant-iT™ Protein Assay Kit
Protein 0.25–5 µg 470/570 1,000 Q33210

Quant-iT™ bulk reagents and kits for >2,000 samples

Product Target Useful range † Ex/Em ‡ No. of assays Cat. No.
Quant-iT™ PicoGreen dsDNA Assay Kit
Quant-iT™ PicoGreen dsDNA Reagent

dsDNA 0.2–100 ng 502/523 2,000 P7589
P7581
Quant-iT™ PicoGreen dsDNA Assay Kit
Quant-iT™ PicoGreen dsDNA Reagent
dsDNA 0.2–100 ng 502/523 2,000 P11496
P11495
Quant-iT™ OliGreen ssDNA Assay Kit
Quant-iT™ OliGreen ssDNA Reagent
ssDNA 1–200 ng 498/518 2,000 O11492
O7582
Quant-iT™ RiboGreen RNA Assay Kit
Quant-iT™ RiboGreen RNA Reagent
RNA 20–1,000 ng 500/525 2,000 R11490
R11491

* Quant-iT™ assays and reagents can also be used with the Qubit® Fluorometer. †The useful range assumes a 1–20 µL sample volume in a 96-well microplate assay. ‡ Excitation (Ex) and emission (Em), in nm, of the Quant-iT™ detection reagent.