MyQubit File Handling
Downloading a .qbt File
To download a .qbt file from the web, first save the file to your PC. Then, transfer the file from your computer to the root directory of your USB drive. Downloading a .qbt file directly to your USB drive may result in unexpected behavior. Make sure you have only one .qbt file on your USB drive before uploading it to the Qubit® 2.0 Fluorometer.
Safe Removal of USB Drive
After creating or editing the contents of a .qbt file, be sure to safely remove the USB drive from your PC before inserting it into the Qubit® 2.0 Fluorometer to upload a new assay. Failure to do so may result in an unexpected outcome.
Uploading Your New Assay(s) to the Qubit® 2.0 Fluorometer
The Qubit® 2.0 Fluorometer will guide you through the process of uploading your new assay(s). Up to four new assays can be created using a single .qbt file, and a total of 20 new assays may be permanently uploaded to the device. The existing Qubit® assays for DNA, RNA, and protein quantification remain unaffected by the presence of any new applications created using MyQubit. Likewise, the workflow and appearance of new assays remain consistent, as does the ability to store and access data. The only thing that changes is the assay identity and target analyte, giving you maximum flexibility in the use of your Qubit® instrument.
To upload your new assay(s) to the Qubit® instrument, first turn the power off by unplugging the device. While the instrument is unplugged, insert the USB drive into the instrument and plug it back in. Your Qubit® 2.0 Fluorometer will recognize if there is a .qbt file present on the USB drive. If the file is correctly populated, you will be prompted to upload the assays contained in the .qbt file (Figure 1). The contents of a .qbt file will be uploaded to the instrument in less than 3 seconds. Once upload is complete, you will be directed to a new Home screen where the Parent Name of your new MyQubit assay will be visible.
Assays can be permanently uploaded without affecting the look or performance of existing applications.
Making Changes to an Existing .qbt File
Any .qbt file that you create may be edited multiple times. However, changes to a MyQubit assay file should first be made externally on a PC and then saved to the USB drive prior to uploading it to the Qubit® 2.0 Fluorometer. Do not save edits directly to the USB drive.
Overwriting an Existing Assay
The Qubit® 2.0 Fluorometer will recognize when you attempt to upload a new assay using MyQubit firmware with a Parent Name that already exists. Name recognition is not case sensitive. You may overwrite assays by following the screen prompts on the instrument (Figure 2).
The Qubit® 2.0 Fluorometer recognizes when you attempt to upload a new assay with a Parent Name that already exists. You may wish to overwrite the existing family of assays. Specific assays under a given Parent Name may not be overwritten. The Qubit® 2.0 Fluorometer recognizes the Parent Name of any given .qbt file as the name of the new assay(s) being uploaded on an individual basis. An existing family of assays may be overwritten based on the equivalence of Parent Names, despite the individual assays in the .qbt file assays being different. You should therefore choose the Parent Name of your .qbt file(s) accordingly.
The exception is with the existing assays for DNA, RNA, and protein quantification. These assays cannot be overwritten. A .qbt file containing a Parent Name of “DNA,” “RNA”, or “Protein” will constitute an error and this file will not be able to be uploaded to the instrument. The name recognition is not case sensitive. Additional assays for the quantification of nucleic acids or protein can be created using MyQubit firmware; however, the Parent Names must be chosen accordingly.
Deleting a New Assay or Assays from the Qubit® 2.0 Fluorometer
To delete an assay or assays from your Qubit® 2.0 Fluorometer, you must upgrade the firmware. With the USB drive inserted into the instrument, press the firmware version button at the top of the graphical display. Make sure that the appropriate firmware upgrade files are present on the USB drive, then press the Update button. If there are any assays present on the device that have previously been uploaded using MyQubit firmware, they will be listed in alphabetical order according to the Parent Name. Here, you may select an assay or family of assays to delete (Figure 3). Individual assays within Parent groupings cannot currently be deleted on their own. Using this procedure, all assays under a given Parent Name will be deleted. After performing the firmware upgrade, the Qubit® 2.0 Fluorometer will open at the Home screen, with the remaining assays still visible
After performing a firmware upgrade, you may choose to delete one or more existing MyQubit assays. This upgrade will not affect instrument performance in any way. You may continue to use the remaining assays according to their respective protocols. The preexisting assays for DNA, RNA, and protein quantification cannot be deleted from the instrument.
Note that the Qubit® 2.0 Fluorometer is not backwards-compatible. Only perform firmware upgrades using the version of firmware currently installed on the instrument, or higher. Do not attempt to upgrade to an older version of firmware—doing so may cause unpredictable instrument behavior.
Note: Firmware versions are numerical in chronology, with newer versions being larger.
Addressing Common Errors Associated with .qbt Files
- Make sure that there is only one .qbt file on the USB drive at a time.
- Make sure that you have successfully upgraded your Qubit® 2.0 Fluorometer to v3.10 firmware.
- Make sure that all necessary fields are filled out in the .qbt file.
- Make sure that all fields are included for each assay in the .qbt file, even if content is not required for your assay.
- Create and edit the .qbt file externally using a PC, then transfer to a USB drive afterwards. Do not attempt to create or edit a .qbt file directly from the USB drive.
- Make sure that the .qbt file is saved to the root directory of the USB drive.
- Make sure that the field entries in your .qbt file adhere to the guidelines described in “The .qbt File.”
- Make sure that the name of your .qbt file does not exceed 20 characters and does not contain any commas.
It is not possible to upload a .qbt file if it contains any errors.
The Instrument Is Full
The Qubit® 2.0 Fluorometer can hold up to 20 new individual assays created using MyQubit firmware. This number can be reached if five .qbt files (five Parent Names) are created and uploaded, each containing the maximum number of four individual assays. However, assays cannot currently be added or deleted on an individual basis; rather, this is done using the designation of Parent Names. Therefore, an instrument may be deemed full if it contains five new Parent Name buttons, even if the total number of individual assays does not exceed 20. If this is the case, you may simply add additional assays under an existing Parent Name using either a new or edited .qbt file.
You will be informed that the Qubit® 2.0 Fluorometer is full upon inserting a USB drive for .qbt file upload if the instrument already contains five Parent Name buttons. You will have the opportunity to replace an existing set of assays if you choose (Figure 6).
If you attempt to upload a new MyQubit assay to the Qubit® 2.0 Fluorometer and the maximum number of assays has already been reached, you will see the above screen. If you do not wish to overwrite an existing assay, press Cancel to exit the overwrite process.