Easy, accurate and sensitive
Qubit® Assay Kits provide concentrated assay reagent, dilution buffer, and prediluted standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 μL and 20 μL is acceptable), and read the concentration using the Qubit® 2.0 Fluorometer. The assay is performed at room temperature, and the signal is stable for 3 hours.
Qubit® assays are ideal when you need to quantitate 1–20 samples. For 20–2,000+ samples, use Quant-iT™ Assay kits and reagents for use with microplate readers.
PLEASE NOTE: All Qubit® Assay Kits except for the microRNA and MyQubit assays are compatible with both the Qubit® 2.0 Fluorometer and the original Qubit® (1.0) Fluorometer. The new microRNA and MyQubit assays are only compatible with the Qubit 2.0 Fluorometer.
Qubit® assay kits selection guide
|Qubit® assay kit||Assay range||Sample starting concentration
|dsDNA BR Assay||2–1000 ng||100 pg/µL–1 ug/µL||Q32850||Q32853|
|dsDNA HS Assay
||10 pg/µL–100 ng/µL
||50 pg/µL–200 ng/µL||Q10212||NA|
|RNA HS Assay
||250 pg/µL –100 ng/µL
|RNA BR Assay
||1 ng/µL–1 µg/µL
|microRNA Assay||1–500 ng||0.05–100 ng/µL||Q32880||Q32881|
||12.5 µg/mL–5 mg/mL
|* Based on an assay volume of 200 μL. NA = Not Available.|
Qubit® assay kits data
Figure 1. Performance of the Qubit® dsDNA HS Assay. The Qubit® dsDNA HS Assay has a linear detection range of 0.2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA.
Figure 2. Performance of the Qubit® RNA Assay. The Qubit® RNA Assay has a linear detection range of 5–100 ng and is selective for RNA, even in the presence of an equal mass of DNA.
Figure 3. The Qubit® Protein Assay has a linear detection range of 0.25-5 µg and is selective for protein, even in the presence of an equal mass of DNA or RNA.
Basic Qubit® fluorometric quantitation protocol
1. Prepare dye Working Solution in a plastic tube.
a. Use 200 µL of buffer for every sample.
b. Use 1 µL of dye for every sample.
c. Mix by vortexing.
2. Aliquot 190 µL of Working Solution into two assay tubes for standards (three for the protein assay).
3. Add 10 µL of each Standard to an assay tube and mix by vortexing.
4. Aliquot 180–199 µL of Working Solution into assay tubes for samples.
a. The assays tolerate 1–20 µL of sample per tube.
b. The final volume in each tube after adding sample should be 200 µL.
5. Add 1–20 µL of each sample to an assay tube and mix by vortexing.
6. Incubate 2 minutes (15 minutes for the protein assay).
7. Read the results in the Qubit® 2.0 Fluorometer.
- Download the Qubit® 2.0 Quick Reference Card