Easy, accurate and sensitive

Qubit® Assay Kits provide concentrated assay reagent, dilution buffer, and prediluted standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 μL and 20 μL is acceptable), and read the concentration using the Qubit® 2.0 Fluorometer. The assay is performed at room temperature, and the signal is stable for 3 hours.

Qubit® assays are ideal when you need to quantitate 1–20 samples.  For 20–2,000+ samples, use Quant-iT™ Assay kits and reagents for use with microplate readers.

PLEASE NOTE: All Qubit® Assay Kits except for the microRNA and MyQubit assays are compatible with both the Qubit® 2.0 Fluorometer and the original Qubit® (1.0) Fluorometer. The new microRNA and MyQubit assays are only compatible with the Qubit 2.0 Fluorometer.

Qubit® assay kits selection guide

Qubit® assay kit Assay range Sample starting concentration
100 Samples*
500 Samples*
dsDNA BR Assay 2–1000 ng 100 pg/µL–1 ug/µL Q32850 Q32853
dsDNA HS Assay
0.2–100 ng
10 pg/µL–100 ng/µL
Q32851
Q32854
ssDNA Assay
1–200 ng
 50 pg/µL–200 ng/µL Q10212 NA
RNA HS Assay
5–100 ng
250 pg/µL –100 ng/µL
Q32852 Q32855
RNA BR Assay
20–1000 ng
1 ng/µL–1 µg/µL
Q10210 Q10211
microRNA Assay 1–500 ng 0.05–100 ng/µL Q32880 Q32881
Protein Assay
0.25–5 µg
12.5 µg/mL–5 mg/mL
Q33211 Q33212
* Based on an assay volume of 200 μL. NA = Not Available.

Qubit® assay kits data

Figure 1. Performance of the Qubit® dsDNA HS Assay. The Qubit® dsDNA HS Assay has a linear detection range of 0.2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA.

Figure 2.  Performance of the Qubit® RNA Assay. The Qubit® RNA Assay has a linear detection range of 5–100 ng and is selective for RNA, even in the presence of an equal mass of DNA.

Figure 3. The Qubit® Protein Assay has a linear detection range of 0.25-5 µg and is selective for protein, even in the presence of an equal mass of DNA or RNA.

Basic Qubit® fluorometric quantitation protocol

1. Prepare dye Working Solution in a plastic tube.
                a.    Use 200 µL of buffer for every sample.
                b.    Use 1 µL of dye for every sample.
                c.    Mix by vortexing.

2. Aliquot 190 µL of Working Solution into two assay tubes for standards (three for the protein assay).

3. Add 10 µL of each Standard to an assay tube and mix by vortexing.

4. Aliquot 180–199 µL of Working Solution into assay tubes for samples.
               a.  The assays tolerate 1–20 µL of sample per tube.
               b.  The final volume in each tube after adding sample should be 200 µL.

5. Add 1–20 µL of each sample to an assay tube and mix by vortexing.

6. Incubate 2 minutes (15 minutes for the protein assay).

7. Read the results in the Qubit® 2.0 Fluorometer.

Ordering information

Sku Name Size Price Qty
Q32866 Qubit® 2.0 Fluorometer 1 each USD 2,035.00
Q32871 Qubit® 2.0 Quantitation Starter Kit 1 kit USD 2,198.00
Q32872 Qubit® 2.0 Quantitation Lab Starter Kit 1 kit USD 8,973.00
Q32850 Qubit® dsDNA BR Assay Kit 100 assays USD 79.00
Q32853 Qubit® dsDNA BR Assay Kit 500 assays USD 237.00
Q32851 Qubit® dsDNA HS Assay Kit 100 assays USD 79.00
Q32854 Qubit® dsDNA HS Assay Kit 500 assays USD 237.00
Q10212 Qubit® ssDNA Assay Kit 1 kit USD 79.00
Q32852 Qubit® RNA HS Assay Kit 100 assays USD 79.00
Q32855 Qubit® RNA HS Assay Kit 500 assays USD 237.00
Q32881 Qubit® microRNA Assay Kit 500 assays USD 241.00
Q10211 Qubit® RNA BR Assay Kit 500 assays USD 237.00
Q33211 Qubit® Protein Assay Kit 100 assays USD 79.00
Q33212 Qubit® Protein Assay Kit 500 assays USD 237.00
Q32856 Qubit® Assay Tubes 500 tubes USD 70.25