Qubit Fluorometric Quantitation

Designed for your precious samples and high-investment applications

The Qubit® 2.0 Fluorometer is our second generation fluorometer designed to work seamlessly with our highly specific and sensitive Qubit® DNA, RNA, and protein quantitation assays.

The new features of this next-generation fluorometer include:

  • Large LCD color touch screen to ensure precision
  • Easy workflow navigation to save time
  • Automatic data logging and USB port for efficient data management
  • Standard curve display after calibration completion

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Download the product brochure: Qubit® 2.0 Fluorometer: For Your Precious Samples and High-value Applications

Who should use the Qubit® 2.0 Fluorometer?

Ask yourself:

  • Are your samples rare and difficult to process?
  • Do you have only small quantities of DNA, RNA or protein after extraction?
  • Is the sample going to be used in expensive downstream experiments?
  • Do you use applications like quantitative real-time PCR (qPCR) that require precise measurements?
  • Will you perform transfection or other applications where it may be days or weeks before you get results?
  • Are you doing complex sample preparations that require special skills, such as laser capture microdissection?  

If you answered YES to any of these questions, then the Qubit® 2.0 Fluorometer is for you.

How does it work?

The Qubit® 2.0 Fluorometer utilizes specifically designed fluorometric technology using Molecular Probes® dyes to quantitate biomolecules of interest. These fluorescent dyes emit signals ONLY when bound to specific target molecules, even at low concentrations.

The color touch screen allows you to easily navigate through the different options (Figure 1).


Figure 1. Qubit® 2.0 Fluorometer sample workflow. 1. In the initial menu, select the desired assay. 2. Follow the instructions to enter each standard tube in the required order. 3. Measure your sample. 4. The Qubit® 2.0 fluorometer can calculate concentrations for you. Just input the volume of sample used and the desired units of concentration.

The Qubit® 2.0 Fluorometer will save your data with the data logging function and allows you  to transfer data using the USB drive. In addition, new features such as showing the standard curve after the calibration makes the Qubit® 2.0 Fluorometer a truly user-friendly instrument (Figure 2).

"Qubit 2.0 standard curve"  

 

Figure 2.  The Qubit® 2.0 Fluorometer displays the standardcurve after completion of the calibration.

Qubit® Assays

The Qubit® assays for use with the fluorometer are all performed using the same general protocol. A simple mix-and-read format is used, with incubation times of only 2 minutes required for DNA and RNA assays (Figure 3).

Quant-iT™ Assays  

 

Figure 3. Performance of the Qubit® dsDNA HS Assay. The Qubit® dsDNA HS Assay has a linear detection range of 0.2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA.

An integrated solution

Qubit® Assay Kits work seamlessly with the Qubit® 2.0 Fluorometer, giving you the highest accuracy and sensitivity, and leading to better results in downstream experiments. Just choose the assay, open the kit, and you're ready to quantitate—all settings and calculations are performed for you in 5 minutes or less.

Simplicity—no need to freeze

Qubit® assays were designed to be performed at room temperature. For best results, store reagent and buffer at room temperature to streamline your workflow.

PLEASE NOTE: All Qubit® Assay Kits except for the microRNA and MyQubit assays are compatible with both the Qubit® 2.0 Fluorometer and the original Qubit® (1.0) Fluorometer. The new microRNA and MyQubit assays are only compatible with the Qubit 2.0 Fluorometer.

Choose your Qubit® 2.0 Fluorometer

Qubit® 2.0 Fluorometer

Qubit® Fluorometer

Includes:

  • 1 Qubit® 2.0 fluorometer
  • 1 Qubit® Power Adapter
  • Qubit® owner's manual
Qubit® Quantitation Starter Kit

Includes:

  • 1 Qubit® 2.0 Fluorometer
  • 500 assay tubes
  • Qubit® dsDNA BR Assay Kit, 100 assays
  • Qubit® dsDNA HS Assay Kit, 100 assays
  • Qubit® RNA Assay Kit, 100 assays
  • Quant-iT® Protein Assay Kit, 100 assays
  • 1 Qubit® Power Adapter
  • Qubit® owner's manual
Qubit®Quantitation Lab Starter Kit

Includes:

  • 5 Qubit® 2.0 Fluorometers
  • Qubit® dsDNA BR Assay Kit, 100 assays
  • Qubit® dsDNA HS Assay Kit, 100 assays
  • Qubit® RNA Assay Kit, 100 assays
  • Qubit® Protein Assay Kit, 100 assays
  • 1 Qubit® Power Adapter
  • Qubit® owner's manual

Ordering information

Catalog # Name Size List Price (USD) Qty
Q32866 Qubit® 2.0 Fluorometer 1 each 2,035.00
Q32871 Qubit® 2.0 Quantitation Starter Kit 1 kit 2,198.00
Q32872 Qubit® 2.0 Quantitation Lab Starter Kit 1 kit 8,973.00
Q32850 Qubit® dsDNA BR Assay Kit 100 assays 79.00
Q32853 Qubit® dsDNA BR Assay Kit 500 assays 237.00
Q32851 Qubit® dsDNA HS Assay Kit 100 assays 79.00
Q32854 Qubit® dsDNA HS Assay Kit 500 assays 237.00
Q10212 Qubit® ssDNA Assay Kit 1 kit 79.00
Q32852 Qubit® RNA HS Assay Kit 100 assays 79.00
Q32855 Qubit® RNA HS Assay Kit 500 assays 237.00
Q10210 Qubit® RNA BR Assay Kit 100 assays 79.00
Q10211 Qubit® RNA BR Assay Kit 500 assays 237.00
Q33211 Qubit® Protein Assay Kit 100 assays 79.00
Q33212 Qubit® Protein Assay Kit 500 assays 237.00
Q32856 Qubit® Assay Tubes 500 tubes 70.25