AbAg IA IVD

Dynabeads® offer many unique benefits and are well established as the gold standard immunodiagnostic method. A comprehensive range of products for use in immunodiagnostics is available, across different sizes and surface chemistries. Some Dynabeads® are optimized for immobilization of peptides and small antigens for antigen-down assays. Other Dynabeads® allow immobilization of larger proteins and antibodies, for use as a solid-phase in traditional immunoassays. Some of the products are from our existing bio-discovery portfolio, while others are developed and supplied on an OEM basis.

Benefits include

  • High capacity & wide dynamic range
  • High signal-to-noise ratio
  • Adaptable to any known detection method & assay methodology
  • Efficient & reproducible ligand immobilization
  • Easy assay development
  • Direct or indirect capture approach
  • No magnetic remanence
  • Excellent kinetics & shorter assay times
  • Reproducible automated behavior
  • Fast and efficient washing
  • Increased sensitivity
  • Long reagent stability on the immunoassay analyzer
  • Easy handling in manufacturing and shortened assay development time
Product Characteristics Selected Applications

Dynabeads®
M-270
Epoxy
 

 

  • Hydrophilic bead surface coating with low non-specific protein binding
  • Freeze dried monosized beads. Easy to disperse in buffer before use
  • Size: 2.8 micrometer
  • Active epoxy groups for direct covalent coupling to antibodies, antigens or other biomolecules . No further surface activation required
  • Low aggregation
  • Immunoassays and immunodiagnostics
  • Protein and peptide purification
  • Immunoprecipation

Dynabeads®
MyOne™
Epoxy
 

 

  • Bead surface chemistry optimized for enhanced binding capacity and low non-specific binding.
  • Freeze dried monosized beads, easy to disperse in buffer before use
  • Size: 1 micrometer
  • Low sedimentation rate and higher reaction kinetics compared to M-270 beads
  • Active epoxy groups for direct covalent coupling to antibodies, antigens or other biomolecules . No further surface activation required
  • Immunoassays and immunodiagnostics
  • Protein and peptide purification
  • Immunoprecipation
Dynabeads® MyOne™
Tosylactivated

 

 

  • Hydrophobic bead surface
  • Size: 1 micrometer, monodisperse
  • Iron content (Ferrites): 26% (37%)
  • Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
  • Active chemical functionality: 0.04-0.06 mmol/g beads
  • Direct covalent binding of proteins/peptides via primary amino- or sulphydryl groups. No further surface activation required.

 

  • Immunoassays and immunodiagnostics
  • Protein and peptide purification
  • Phage display and biopanning
  • Cell separation
Dynabeads®
M-280
Tosylactivated
  • Hydrophobic bead surface with p-toluene-sulfonyl (tosyl) groups
  • Size: 2.8 micrometer, monodisperse
  • Iron content (Ferrites): 12% (17%)
  • Active chemical functionality: 0.1-0.2 mmol/g beads
  • Direct covalent binding of proteins/peptides via primary amino- or sulphydryl groups. No further surface activation required.
  • Immunoassays and immunodiagnostics
  • Protein and peptide purification
  • Phage display and biopanning
  • Cell separation
 

Dynabeads® MyOne™
Streptavidin T1

  • Hydrophobic bead surface
  • Based on tosylactivated beads (T1)
  • Size: 1 micrometer, monodisperse
  • BSA as blocking protein
  • Isoelectric point: pH 5.0
  • Low charge (-10 mV, at pH 7)
  • Iron content (Ferrites): 26% (37%)
  • Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
  • Free biotin binding: > 1,100-1,700 pmoles/mg beads
  • Binding biotinylated Ig: 40-50 µg/mg beads
  • Immunoassays and immunodiagnostics
  • Isolation of biotinylated molecules
  • Protein and peptide purification
  • Phage display and biopanning
  • Cell separation

Dynabeads®
M-280
Streptavidin

  • Hydrophobic bead surface
  • Based on tosylactivated beads
  • Size: 2.8 micrometer, monodisperse
  • BSA as blocking protein
  • Isoelectric point: pH 5.0
  • Low charge (-10 mV, at pH 7)
  • Iron content (Ferrites): 12% (17%)
  • Free biotin binding: 650-900 pmoles/mg beads
  • Binding biotinylated Ig: 5-10 µg/mg beads
  • Immunoassays and immunodiagnostics
  • Isolation of biotinylated molecules
  • Protein and peptide purification
  • Phage display and biopanning
  • Cell isolation
Dynabeads®
M-280
Sheep
anti-Rabbit IgG
  • Hydrophobic bead surface
  • Coated with affinity purified sheep polyclonal against all rabbit IgG subclasses.
  • Size: 2.8 micrometer, monodisperse
  • Iron content (Ferrites): 12% (17%)
  • Mostly Fc reactive
  • Binding capacity: 0.1-1 µg Ig/107 beads

 

  • Immunoassays and immunodiagnostics
  • Protein and peptide purification
  • Phage display and biopanning
  • Cell isolation

 

Dynabeads®
M-270
Streptavidin
  • Hydrophilic bead surface
  • Based on carboxylic acid beads
  • Size: 2.8 micrometer, monodisperse
  • No blocking proteins used
  • Isoelectric point: pH 4.5
  • Highly charged (-50 mV, at pH 7)
  • Iron content (Ferrites): 14% (20%)
  • Low aggregation of beads in high salt solutions
  • Free biotin binding: 650-1,350 pmoles/mg beads
  • Binding biotinylated Ig: 5-10 µg/mg beads
  • Immunoassays and immunodiagnostics with hydrophobic targets
  • Isolation of biotinylated molecules
  • Sequence specific DNA/RNA capture in nucleic acid based diagnostics
  • For use in protocols that require GTC lysis or high salt concentrations, or protocols not compatible with BSA.
Dynabeads®
M-270
Carboxylic Acid
  • Hydrophilic bead surface with carboxylic acid groups
  • Size: 2.8 micrometer, monodisperse
  • Iron content (Ferrites): 14% (20%)
  • Very low aggregation
  • Active chemical functionality 150 µmol/g beads
  • Immunoassays and immunodiagnostics
  • Sequence specific DNA/RNA capture in nucleic acid based diagnostics
  • Immobilization of amino-modified oligonucleotides, antibodies or other ligands, for capture of specific DNA/RNA sequences, proteins or any other analyte
Dynabeads®
MyOne™
Streptavidin C1
  • Hydrophilic bead surface
  • Based on carboxylic acid beads (C1)
  • Size: 1 micrometer, monodisperse
  • No blocking proteins used
  • Tween 20 in the buffer
  • Isoelectric point: pH 5.2
  • Medium charged (-35 mV, at pH 7)
  • Iron content (Ferrites): 26% (37%)
  • Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
  • Very low aggregation
  • Free biotin binding: > 2,500 pmoles/mg beads
  • Binding biotinylated Ig: 20-25 µg/mg beads
  • Immunoassays and immunodiagnostics
  • Isolation of biotinylated molecules
  • Sample preparation of proteins for mass spectrometry
  • Sequence specific DNA/RNA capture in nucleic acid based diagnostics