Dynabeads Nucleic Acid IVD

Dynabeads® offer many unique benefits and are widely used for specific capture and detection of nucleic acids in clinical samples. Some of the products are from our existing bio-discovery portfolio, while others are developed and supplied on an OEM basis. Dedicated kits have been developed for isolation of viral nucleic acids, and genomic DNA.

Benefits include:

  • High capacity & wide dynamic range
  • High signal-to-noise ratio
  • Adaptable to any known detection method & assay methodology
  • No magnetic remanence
  • Reproducible automated behavior without mixing
  • Fast and efficient washing
  • Increased sensitivity
  • Easy handling in manufacturing and shortened assay development time
Product Characteristics Selected Applications
Dynabeads® MyOne™
SILANE
  • Monosized beads with an optimized silica-like surface chemistry
  • Highly reproducible binding of both DNA and RNA
  • High specific surface area
    (approx. 20 m2/g)
  • Size: 1 micrometer, monodisperse
  • Isoelectric point: pH 3.5
  • Increased iron content compared to the traditional Dynabeads® MyOne™ product range, and hence a better mobility to magnet
  • Low sedimentation rate and favorable reaction kinetics
  • Flexible starting volumes
  • Isolation of total nucleic acids (DNA/RNA)
  • From biological samples (serum/plasma, human whole blood etc.)
  • Well suited for automated applications
  • Validation and customization (bead, buffer, protocol or format) available upon request.
  • Adjusted to customer requirements on a custom OEM basis
Dynabeads®
SILANE
viral NA
  • Contains Dynabeads® MyOne™ SILANE (see above for characteristics), buffers and a protocol optimized for isolation of viral DNA/RNA
  • High sensitivity for viral DNA and RNA. E.g. can detect HBV DNA in a 200 µl serum sample containing only 40 infectious units
  • Isolation of viral DNA/RNA from human serum/plasma samples
  • Effective isolation of nucleic acids even from challenging viruses (e.g. HBV)
Dynabeads®
SILANE
genomic DNA
  • Contains Dynabeads® MyOne™ SILANE (see above for characteristics), buffers and a protocol optimized for isolation of genomic DNA
  • High integrity and purity of isolated DNA
  • Typically isolates 10 µg DNA from a 350 µl blood sample using 50 µl (2 mg) Dynabeads
  • Linear range of DNA yield relative to White Blood Cell (WBC) counts
  • Isolation of genomic DNA from human whole blood
  • From fresh/frozen blood samples, and with a wide range of anticoagulants
Dynabeads® Oligo(dT)25
  • Hydrophilic bead surface
  • Size: 2.8 micrometer
  • Iron content (Ferrites): 14% (20%)
  • Amount of coupled oligo-dT25 probe:
    70 pmol/mg beads
  • Capture of polyA mRNA:> 2 µg/mg beads

 

  • Capture of polyA+ mRNA directly from crude starting samples
  • Build a solid-phase cDNA library
  • Specific capture of nucleic acid targets (using capture probes with oligo-dA tail)

 

Dynabeads®
M-270
Streptavidin
  • Hydrophilic bead surface
  • Based on carboxylic acid beads
  • Size: 2.8 micrometer, monodisperse
  • Isoelectric point: pH 4.5
  • Highly charged (-50 mV, at pH 7)
  • Iron content (Ferrites): 14% (20%)
  • Low aggregation of beads in high salt solutions
  • Free biotin binding: 650-1,350 pmoles/mg beads
  • Binding biotinylated Ig: 5-10 µg/mg beads

 

  • Isolation of biotinylated molecules
  • Sequence specific DNA/RNA capture in nucleic acid based diagnostics
  • Preparation of single-stranded DNA.
  • For use in protocols that require GTC lysis or high salt concentrations, or protocols not compatible with BSA.
  • Immunodiagnostics with hydrophobic targets

 

Dynabeads®
M-280
Streptavidin

  • Hydrophobic bead surface
  • Based on tosylactivated beads
  • Size: 2.8 micrometer, monodisperse
  • BSA as blocking protein
  • Isoelectric point: pH 5.0
  • Low charge (-10 mV, at pH 7)
  • Iron content (Ferrites): 12% (17%)
  • Free biotin binding: 650-900 pmoles/mg beads
  • Binding biotinylated Ig: 5-10 µg/mg beads
  • Isolation of biotinylated molecules
  • Sequence specific DNA/RNA capture in nucleic acid based diagnostics
  • Purification of DNA/RNA binding proteins
  • Protein purification, phage display and biopanning
  • Immunodiagnostics
Dynabeads® MyOne™
Streptavidin C1
  • Hydrophilic bead surface
  • Based on carboxylic acid beads (C1)
  • Size: 1 micrometer, monodisperse
  • No blocking proteins used
  • Tween 20 in the buffer
  • Isoelectric point: pH 5.2
  • Medium charged (-35 mV, at pH 7)
  • Iron content (Ferrites): 26% (37%)
  • Low sedimentation rate and improved reaction kinetics compared to
    M-280/M-270 beads
  • Very low aggregation
  • Free biotin binding: > 2,500 pmoles/mg beads
  • Binding biotinylated Ig: 20-25 µg/mg beads

 

  • Isolation of biotinylated molecules
  • Sequence specific DNA/RNA capture in nucleic acid based diagnostics
  • Preparation of single-stranded DNA
  • High throughput nucleic acid clean-up protocols
  • Well suited for automated applications.

 

Dynabeads® MyOne™
Carboxylic Acid
  • Hydrophilic bead surface with carboxylic acid groups
  • Size: 1 micrometer, monodisperse
  • Iron content (Ferrites): 26% (37%)
  • Low sedimentation rate and improved reaction kinetics compared to
    M-280/M-270 beads
  • Very low aggregation
  • Active chemical functionality: 0.6 mmol/g beads
  • Coupling capacity: 20-25 µg proteins or 700 pmol oligonucleotides per mg beads

 

  • Immobilization of amino-modified oligonucleotides, antibodies or other ligands, for capture of specific DNA/RNA sequences, proteins or any other analyte
  • Well suited for automated applications

 

Dynabeads®
M-270
Carboxylic Acid
  • Hydrophilic bead surface with carboxylic acid groups
  • Size: 2.8 micrometer, monodisperse
  • Iron content (Ferrites): 26% (37%)
  • Very low aggregation
  • Active chemical functionality 150 µmol/g beads
  • Immobilization of amino-modified oligonucleotides, antibodies or other ligands, for capture of specific DNA/RNA sequences, proteins or any other analyte
  • Well suited for automated applications
Dynabeads® MyOne™
Tosylactivated

 


  • Hydrophobic bead surface
  • Size: 1 micrometer, monodisperse
  • Iron content (Ferrites): 26% (37%)
  • Low sedimentation rate and improved reaction kinetics compared to
    M-280/M-270 beads
  • Active chemical functionality: 0.04-0.06 mmol/g beads
  • Immobilization via primary amino- or sulphydryl groups.
  • Protein purification, phage display and biopanning
  • Immunodiagnostics
Dynabeads®
M-280
Tosylactivated
  • Hydrophobic bead surface with
    p-toluene-sulfonyl (tosyl) groups
  • Size: 2.8 micrometer, monodisperse
  • Iron content (Ferrites): 12% (17%)
  • Active chemical functionality: 0.1-0.2 mmol/g beads
  • Immobilization via primary amino- or sulphydryl groups
  • Protein purification, phage display and biopanning
  • Immunodiagnostics