TaqPath™ qPCR Master Mixes, CG
Confidence and compliance for diagnostic development and testing
With superior quality, excellent performance, and regulatory compliance, TaqPath™ CG master mixes are designed to deliver confidence and performance for even your most demanding applications. With 15 years of technology leadership in real-time PCR, Life Technologies is committed to providing clinical laboratories with the trusted, versatile, and innovative tools for molecular diagnostic development and testing.
TaqPath™ qPCR products
TaqPath™ qPCR master mixes have been validated to deliver exquisite lot-to-lot reproducibility for absolute CT consistency and dynamic range across a wide variety of assays and applications. These general purpose reagents are manufactured under stringent controls, including change control, document control, and purchasing controls. In addition, each batch is quality-control tested prior to release. TaqPath™ qPCR master mixes enable superior performance, improved reproducibility, and traceability.
TaqPath™ qPCR Master Mix, CG is a 2X formulation designed for gene expression and miRNA analysis containing thermostable fast DNA polymerase and dNTPs in an optimized buffer solution for maximum robustness and reproducibility.
Compatible with both standard and fast cycling modes, TaqPath™ qPCR Master Mix, CG has been engineered for enhanced stability, allowing your preassembled reactions to be left at room temperature for more than 24 hours without impacting performance. In addition, this product has been rigorously validated for duplex performance with both endogenous and exogenous controls, and is duplex performance tested for each manufacturing lot.
Through extensive benchmarking of this formulation against other leading suppliers' standard and fast master mixes, we are confident TaqPath™ qPCR Master Mix, CG will consistently deliver best-in-class sensitivity, accuracy, dynamic range, and specificity.
TaqPath™ 1-Step RT-qPCR Master Mix, CG is a 4X formulation designed for 1-step processing of RNA and DNA targets for pathogen detection, or in 1-step gene expression workflows.
Ease of use
This formulation combines thermostable MMLV reverse transcriptase, dNTPs, and fast DNA polymerase all in a single-tube formulation, so all you have to do is add your sample, target-specific primers, and probes.
Optimized to tolerate inhibitors commonly found in clinical samples and deliver superior sensitivity, specificity, and dynamic range with multiplexing compatibility, TaqPath™ 1-Step RT-qPCR MasterMix, CG is the ideal solution for your most challenging applications.
Every TaqPath™ qPCR master mix has been validated to deliver lot-to-lot reproducibility for absolute CT consistency and dynamic range across a wide variety of assays and applications:
- Gene Expression
- MicroRNA (miRNA)
- Virus/Pathogen Detection
TaqPath™ compliant grade qPCR master mixes enable superior performance, improved reproducibility, and traceability.
Q: Which cycling modes are TaqPath™ qPCR master mixes, CG compatible with?
A: TaqPath™ qPCR master mixes, CG are compatible with both standard and fast cycling modes. Please refer to the product manual and your instrument guide for recommended cycling protocols.
Q: How stable are TaqPath™ qPCR master mixes, CG?
A: TaqPath™ qPCR master mixes, CG have been engineered for enhanced stability, allowing your preassembled reactions to be left at room temperature for more than 24 hours without impacting performance. In contrast, it is recommended for the user to run assembled reactions using TaqPath™ 1-Step RT-qPCR Master Mix, CG immediately after reaction set up.
Q: Are the TaqPath™ qPCR master mixes, CG UDG/UNG-compatible?
A: TaqPath™ qPCR master mixes, CG contain dUTP and UDG/UNG to mitigate potential risks of amplicon cross-contamination.
Q: What is the storage condition for TaqPath™ qPCR master mixes, CG?
A: The recommended storage for TaqPath™ qPCR master mixes, CG is 4°C, and the recommended storage for TaqPath™ 1-Step RT-qPCR Master Mix, CG is -20°C.
Q: How many reactions can I run using 1 package of the product?
A: It depends on a volume of your reactions. For a standard 20µl reaction, The TaqPath™ qPCR Master Mix, CG, is a 2X mix, so a 5mL vial is sufficient to run 500 reactions. In contrast, the TaqPath™ 1-Step RTqPCR Mix is a 4x mix so a 5mL vial is sufficient to run 1000 reactions.
TaqPath™ qPCR master mixes, CG
Take 50% off your TaqPath™ qPCR master mix now. Reference promotion code REV001
*This promotion is open to customers in the US only. Discount will apply to qualifying orders received by Life Technologies no later than June 30, 2014, or until promotional supplies are depleted, whichever comes first. Discount only applies to a maximum of 1 kit per customer. Discount-eligible items are A15297, A15298, A15299, A15300, A16245, A16247, and A16248. Discount applies to the list price in effect at the time the order is received by Life Technologies. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.