Blog: AmpFlSTR® Yfiler® Kit Drives the Success of Y Chromosome Analysis in Forensic Genetics

HID in Action

Y Chromosome Analysis - In 1992, a paper was published describing the first case to be successfully resolved using the technique of Y chromosome analysis; in a murder case in Berlin, a male-female mixture could not be solved using autosomal analysis but analysis of just one locus on the Y chromosome was able to clearly exclude a male suspect.Read More

Date Posted: 05/15/12

Blog: The Basics of RNA Preparation

Preparing high-quality RNA

RNA Technologies and Techniques - A number of RNA preparation technologies are widely available that can be classified into four general techniques: organic extraction methods, spin basket formats, magnetic particle methods, and direct lysis methods. While all can be used to prepare high-quality RNA suitable for a wide variety of analysis techniques, there are several factors to consider in selecting the right purification technology.Read More

Date Posted: 05/14/12

Blog: A Guide to Understanding Journal Phraseology

Friday fun!

What Were They Thinking? - Have you ever wondered what the authors of scientific journals are thinking or trying to reiterate? Here are some popular phrases often found in scientific journals with our interpretation of what the authors might have been thinking when they wrote them.Read More

Date Posted: 05/11/12

Blog: Rare Stem Cells Detected Using the Attune® Acoustic Focusing Cytometer

Putative Limbal Stem Cells in Populations of Normal Human Corneal Epithelial Cells

Rare Stem Cells Identified - It is widely accepted that the stem cells which maintain and renew the corneal epithelium reside in the limbus, located on the periphery of the cornea. They are commonly referred to as limbal stem cells (LSCs) and are useful for basic research into various aspects of corneal biology such as ocular injuries that impair vision.Read More

Date Posted: 05/10/12

Blog: The How's, What's, and Why's of Gene Expression Assay Design

Exon, specificity, names, and assay ID suffixes

Gene Expression Assays - Life Technologies offers more than 1.3 million predesigned TaqMan® Gene Expression Assays. Learn the how's, what's and why's of gene expression assay design to help you make the right decision when selecting assays for your research. Read More

Date Posted: 05/09/12

Blog: Vector vs. Viral RNAi Delivery

Choosing RNAi Delivery Methods

In Vitro or In Vivo? - Although employing RNAi vector systems can be slightly more involved than using synthetic RNAi reagents, the flexibility of the vector based systems is compelling for many RNAi researchers conducting both in vitro and in vivo experiments.Read More

Date Posted: 05/08/12

Blog: Gibco Hero for the Month of June

Gibco 50th Anniversary

Congratulations Gibco Hero of the Month Dr. Yamanaka! - We were honored to interview the father of induced pluripotent stem cells (iPSCs), about his journey and what he sees in the future for stem cell research.Read More

Date Posted: 05/07/12

Blog: Interview with Dr. Victor Ambros About miRNAs

How Lin-4 was discovered (miRNAs)

Dr. Victor Ambros - received his Ph.D. from MIT in 1979 and now serves as a Professor of Genetics at Dartmouth Medical School. The goal of the Ambros laboratory is to further the understanding of the genetic and molecular mechanisms underlying the temporal control of development using C. elegans larva as a model. The laboratory also uses Drosophila melanogaster to explore problems in developmental timing and the roles of microRNAs (miRNAs) in animal development. Using genetic approaches they hope to determine the functions of the miRNAs, to identify potential antisense target mRNAs, and to characterize the consequences of their regulatory interactions.Read More

Date Posted: 05/07/12

Blog: Bubbles in Mounted Slides?

How to prevent bubbles on mounted slides

Tiny Bubbles - great for a song title, but not so great for wet slides. Avoid air bubbles in the coverslips of mounted slides by learning why wet slides get bubbles and tips for preventing them.Read More

Date Posted: 05/02/12

Blog: Integration-Free iPS Cells with a Single Transduction & Streamlined Workflow

Easy, fast, streamlined iPSC generation

Dr. Uma Lakshmipathy a Sr. Staff Scientist, Biology presented work - on the creation of integration-free induced pluripotent stem cells with the CytoTune™ -iPS Reprogramming Kit. This kit provides an extremely streamlined process for the generation of induced pluripotent stem cells. The four Yamanaka factors (Oct3⁄4, Sox2, Klf4, and cMyc) included in the kit can be transduced overnight creating a simple efficient workflow. Read More

Date Posted: 05/01/12