Blog: Detecting Marine Photosynthetic Picoplankton with Flow Cytometry

Environmental Microbiology

Flow Cytometry - is a powerful tool for studying the biology, ecology, and biogeochemistry of marine photosynthetic picoplankton. These organisms are intrinsically fluorescent due to their photopigment content, and differences in photopigment composition can be used to distinguish the various groups.Read More

Date Posted: 04/30/12

Blog: Real-Time qRT-PCR Troubleshooting - Top Ten Most Common Pitfalls

RT-qPCR Tech Tips

Need Help With RT-qPCR? - Having problems with your gene expression or SNP genotyping experiments? Do your amplification curves look sigmoidal? No fear. Here are 10 common pitfalls you may face during RT-qPCR.Read More

Date Posted: 04/26/12

Blog: Human Cell Engineering: Cellular Reprogramming and Genome Editing

Download this paper for free!

Developments in Cell Reprogramming - The recent success in reprogramming somatic cells into induced pluripotent stem cells that are self-renewable in culture, coupled with our increasing ability to effect precise and pre-designed geonomic editing, now readily permits cellular changes at both the genetic and epigenetic levels. Read More

Date Posted: 04/24/12

Blog: Green Biotech Products for Eco Friendly Labs

Life Technologies products go green

Sunday is Earth Day - and Life Technologies is happy to be one of the green biotechnology companies in the Life Sciences industry. With Earth day coming up this weekend, it seemed fitting to highlight the changes we've made to our products to be more eco friendly. Find out what we've changed to offer green biotech products that will help you reduce waste and your lab's footprint on Earth day and everyday thereafter. Read More

Date Posted: 04/20/12

Blog: The Challenges of Blood RNA Isolation

Easy to collect, difficult to treat

Blood RNA - Because blood can be collected using relatively noninvasive procedures and is rapidly regenerated, blood is one of the only human sample types that is routinely available for RNA expression studies and biomarker discovery. As a result, blood is the most commonly used sample type in clinical research, yet it presents several challenges for RNA isolation.Read More

Date Posted: 04/19/12

Blog: Understanding the Phenomenon of Fluorescence

Effectively Using Fluorophore

The Basic Fluorescence Properties of a Fluorophore - excitation and emission are often presented in the form of line graphs. These curves describe the likelihood that excitation and emission will occur as a function of wavelength and provide important information about the expected behavior of the irradiated fluorophore. Understanding the information is an important step in understanding the phenomenon of fluorescence. Read More

Date Posted: 04/18/12

Blog: Basics of Real-Time PCR

What is Real-Time PCR and why to use it

Real-Time PCR—Also Called Quantitative Polymerase Chain Reaction (qPCR) - is one of the most powerful and sensitive gene analysis techniques available and is used for a broad range of applications including quantitative gene expression analysis, genotyping, SNP analysis, pathogen detection, drug target validation and for measuring RNA interference. Frequently, real-time polymerase chain reaction is combined with reverse transcription to quantify messenger RNA (mRNA) and MicroRNA (miRNA) in cells or tissues.Read More

Date Posted: 04/17/12

Blog: Need Cell Culture Help?

Life Technologies has comprehensive cell culture resources to support your research

Cell Culture Videos, Handbook and Protocols - Life Technologies wants to see you be successful with your cell culture research. For this reason, multiple resources in the form of videos, a handbook, and basic cell culture protocols are available on our website to assist you. These videos, the handbook and protocols are...Read More

Date Posted: 04/16/12

Blog: Richard Fekete, Ph.D. Discusses Single Cell Analysis in an Insightful Webinar

Free single cell analysis webinar

Single Cell Analysis with Cells-to-CT Kit - We all know that tissues are composed of heterogeneous mixtures of cells. But what does that mean when we take gene expression measurements from homogenized samples? Are we accurately accounting for the small but critical changes occurring in individual cells? Single-cell analysis can be critical in applications such as candidate drug screening, cell differentiation and stem cell studies, and measuring individual cell responses to specific stimuli. Learn more about single cell analysis in this blog post and in our free webinar recently released.Read More

Date Posted: 04/13/12

Blog: The Fluorescence Light Spectrum, Fluorophore Excitation and Emission

What is Fluorescence?

An Introduction to Fluorescence - Today, many of the favored dyes and stains have a fluorescent component, because fluorescent molecules can be detected with extraordinary sensitivity and selectivity. Fluorescence is the result of a process that occurs in certain molecules (generally polyaromatic hydrocarbons or heterocycles) called fluorophores or fluorescent dyes. The fluorophores and fluorescent dyes absorb and emit light - a process called fluorescence that...Read More

Date Posted: 04/12/12