Blog: Is Pooling Small Interfering RNAs (siRNAs) a Good Idea or Not?

Expert siRNA analysis with Susan Magdaleno, PhD with Life Technologies.

Pooling siRNAs - Susan Magdaleno, PhD, is a senior research and development manager at Life Technologies with experience pooling small interfering RNAs (siRNAs). The big question: Is pooling a good idea or not? Here, Magdaleno provides her expert analysis. Read More

Date Posted: 04/09/12

Blog: Interview with Dr. Victor Ambros About miRNAs

How Lin-4 was discovered (miRNAs)

Dr. Victor Ambros - received his Ph.D. from MIT in 1979 and now serves as a Professor of Genetics at Dartmouth Medical School. The goal of the Ambros laboratory is to further the understanding of the genetic and molecular mechanisms underlying the temporal control of development using C. elegans larva as a model. The laboratory also uses Drosophila melanogaster to explore problems in developmental timing and the roles of microRNAs (miRNAs) in animal development. Using genetic approaches they hope to determine the functions of the miRNAs, to identify potential antisense target mRNAs, and to characterize the consequences of their regulatory interactions.Read More

Date Posted: 05/07/12

Blog: Tips For Isolating High-Quality Total RNA

Isolating Total RNA

Isolating High-Quality Total RNA - A few tips for isolating high-quality total RNA from a wide variety of samples including animal or plant cells, tissue, blood, bacteria, yeast, and liquid samples. Once purified, the total RNA is suitable for use in a variety of downstream applications such as qRT-PCR, northern blotting, nuclease protection assays, RNA amplification for microarray analysis or cDNA library preparation after poly(A)+ selection. Read More

Date Posted: 04/05/12

Blog: The Basics of RNA Preparation

Preparing high-quality RNA

RNA Technologies and Techniques - A number of RNA preparation technologies are widely available that can be classified into four general techniques: organic extraction methods, spin basket formats, magnetic particle methods, and direct lysis methods. While all can be used to prepare high-quality RNA suitable for a wide variety of analysis techniques, there are several factors to consider in selecting the right purification technology.Read More

Date Posted: 05/14/12

Blog: The Challenges of Blood RNA Isolation

Easy to collect, difficult to treat

Blood RNA - Because blood can be collected using relatively noninvasive procedures and is rapidly regenerated, blood is one of the only human sample types that is routinely available for RNA expression studies and biomarker discovery. As a result, blood is the most commonly used sample type in clinical research, yet it presents several challenges for RNA isolation.Read More

Date Posted: 04/19/12