Blog: Three States of Fluorophore During Fluorescence

Three States of Fluorophore During Fluorescence
Fluorescence-based techniques - are valuable tools for studying cellular structure and function, and interactions of molecules in biological systems. Fluorescence is also important in the detection and quantitation of nucleic acids and proteins in gel electrophoresis, microarrays, and fluorescence spectroscopy.Read More

Date Posted: 03/23/12

Blog: The Fluorescence Light Spectrum, Fluorophore Excitation and Emission

What is Fluorescence?

An Introduction to Fluorescence - Today, many of the favored dyes and stains have a fluorescent component, because fluorescent molecules can be detected with extraordinary sensitivity and selectivity. Fluorescence is the result of a process that occurs in certain molecules (generally polyaromatic hydrocarbons or heterocycles) called fluorophores or fluorescent dyes. The fluorophores and fluorescent dyes absorb and emit light - a process called fluorescence that...Read More

Date Posted: 04/12/12

Blog: Understanding the Phenomenon of Fluorescence

Effectively Using Fluorophore

The Basic Fluorescence Properties of a Fluorophore - excitation and emission are often presented in the form of line graphs. These curves describe the likelihood that excitation and emission will occur as a function of wavelength and provide important information about the expected behavior of the irradiated fluorophore. Understanding the information is an important step in understanding the phenomenon of fluorescence. Read More

Date Posted: 04/18/12