Human Cell Engineering: Cellular Reprogramming and Genome Editing
by Jennifer Hornstein - 04/24/12
Download a human cell engineering paper now available.
The recent success in reprogramming somatic cells into induced pluripotent stem cells that are self-renewable in culture, coupled with our increasing ability to effect precise and pre-designed geonomic editing, now readily permits cellular changes at both the genetic and epigenetic levels.
These dual capabilities thus also make possible the generation of genetically-matched, disease-free stem cells from patients for regenerative medicine.
In this paper, Prashant Mali and Linzhao Cheng review key developments in cellular reprogramming and engineering, as well as discuss unresolved issues and future research including:
- An overview of recent developments in iPSC generation including new reprogramming techniques and novel reprogramming factors
- A review of genetic engineering techniques such as homologous recombination and zinc-finger nucleases
- An outline of potential future research directions that will help progress human cell engineering
About the Authors:
Dr. Prashant Mali is a postdoctoral fellow in the Department of Genetics at Harvard Medical School.
Dr. Linzhou Cheng is the Associate Professor of the Stem Cell Program in the Institute of Cell Engineering at the Johns Hopkins University School of Medicine.
Download this human cell engineering paper.