Should You Dilute cDNA for Real-time PCR?
by Life Technologies Sr. Field Application Specialist, Doug Rains - 03/05/13
1:5 dilution? 1:3? 1:10? Or is diluting cDNA for Real-time PCR a waste of time, effort and perfectly good H2O?
In this video, Life Technologies Sr. Field Application Specialist Doug Rains examines whether or not qPCR end-users doing gene expression should dilute cDNA prior to running samples.
This video also looks at the possible need to dilute RNA prior to reverse transcription. This takes into consideration a host of scenarios including final reaction volume and expression level of genes.
Do you have real-time PCR questions? Just ask TaqMan®!