Codon optimization improves gene expression capabilities without changing the polypeptide sequence.  There are 61 codons encoding 20 amino acids. Different expression hosts prefer certain codons.  By changing the codons of the inserted gene to those preferred by the cell factory or expression host, protein production can be improved significantly, sometimes by 100-fold or beyond.

Further, reliability of transgene expression significantly increases.  Codon optimization is used for recombinant gene expression in virtually all known cell systems.  It is used for basic research, bioproduction, vaccine and therapeutics development, cellular screening systems, metabolic engineering, and synthetic biology.

In addition to codon choice, our multiparameter gene optimization algorithm takes into account GC content, mRNA secondary structure, splicing, and more.

Did you know you can perform your own optimization using an online optimization tool?  Visit the gene optimization page to learn more. 

About the Author
Professor Ralf Wagner is the Vice President, Life Technologies Synthetic Biology in Europe