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PCR DNA

Ever wonder what the top 5 DNA FAQ's are? Get the answers from the award wining Life Technologies support team? Here is No. 4:

4. How much PCR product should I add for an optimal cloning reaction?

  • TOPO® and Gateway® cloning: 1:1 molar ratio of insert to vector
  • Restriction enzyme/ligase cloning: 3:1 molar ratio of insert to vector


You can use the following formula to determine the amount of insert needed to give the recommended molar ratio:

x ng insert = (molar ratio)(# bp insert)(ng vector) / (# bp vector)

Interested in all top 5 DNA FAQ's? Get the answers (pg.7) and much more in the new Spring Life In The Lab out now.

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