Ever wonder what the top 5 DNA FAQ's are? Get the answers from the award wining Life Technologies support team? Here is No. 4:
4. How much PCR product should I add for an optimal cloning reaction?
- TOPO® and Gateway® cloning: 1:1 molar ratio of insert to vector
- Restriction enzyme/ligase cloning: 3:1 molar ratio of insert to vector
You can use the following formula to determine the amount of insert needed to give the recommended molar ratio: