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Master Mix

Master mixes

The AgPath-ID™ One-Step RT-PCR Reagents, Path-ID™ qPCR Master Mix, and Path-ID™ Multiplex One-Step RT-PCR Kit have been specifically designed for animal sample matrices. Their development and design is focused on analytical sensitivity.

These products are:

  • Versatile—can be used with a wide range of primers, probes, and thermal cyclers
  • Fast—obtain results in approximately 1 hour

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid, single-tube real-time reverse transcription PCR (RT-PCR) strategy.

Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan® probe-based real-time PCR amplification of DNA targets, optimized for research of importance in animal health and to function in the presence of PCR inhibitors. With the exception of a PCR primer/TaqMan® probe mix for your target, the Path-ID™ qPCR Master Mix includes everything you need for real-time PCR amplification, including:

  • AmpliTaq Gold® ultrapure thermostable DNA polymerase designed for automatic hot start, to minimize nonspecific PCR products
  • Optimized buffer and dNTP concentration, for reliable PCR
  • Passive internal reference, based on proprietary ROX™ dye, for precise data analysis

Path-ID™ Multiplex One-Step RT-PCR Kit is designed for the sensitive, robust amplification and multiplex quantitation of up to 4 RNA targets simultaneously using a simple real-time RT-PCR strategy. The kit includes the buffers and enzymes needed for RT-PCR; you supply only the RNA sample, PCR primers and TaqMan® probes.

Regulatory requirements vary by country; products may not be available in your geographic area.

Sku Name Size Price Qty
4387391M AgPath-ID™ One-Step RT-PCR Reagents with manual 1,000 reactions USD 1,430.00
4388643M Path-ID™ qPCR Master Mix with Manual 100 reactions USD 136.00
4442137 Path-ID™ Multiplex One-Step RT-PCR Kit 1,000 reactions USD 2,055.00