Improving upon innovations in buffer formulation made during the development of earlier AmpFℓSTR® Kits such as the MiniFiler™ Kit, the Identifiler® Plus Kit has been designed to help overcome high levels of PCR inhibition, and promote a more robust and efficient amplification. The new formulation helps deliver results from samples which could not be analyzed using the previous generation Identifiler® Kit.
Figure 1. A comparison of the performance of the Identifiler® Plus Kit with the Identifiler® Kit on an inhibited casework sample. The Identifiler® Kit is unable to amplify a blood sample from a leather glove. The Identifiler® Plus kit delivers a full profile as a result of its enhanced formulation. 1ng input DNA and 28 cycles of amplification were used for both kits.
Figure 2. A study demonstrating the performance of the Identifiler® Plus Kit in the presence of increasing concentrations of common PCR inhibitors hematin and humic acid. Even at the highest concentrations, the Identifiler® Plus Kit shows the ability to recover full profiles. 1ng input DNA and 28 cycles of amplification were used for all replicates.