DNA quantification solutions

Quantifiler® Trio
Quantifiler® HP
HID Real-Time PCR Analysis Software v1.2

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Our advanced quantification kits enable forensic labs to achieve accurate results in less time, for even the most challenging casework samples. The kits evaluate sample quality and quantity in a single reaction.

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The Quantifiler® HP DNA Quantification Kit enables forensic laboratories to simultaneously obtain a quantitative and qualitative assessment of total human DNA in a single, highly sensitive real-time PCR reaction. This guides selection of the optimal short tandem repeat (STR) analysis chemistry (autosomal or miniSTR) and streamlines the workflow while increasing downstream analysis success rates.

The Quantifiler® Trio kit has all the enhanced capabilities of Quantifiler® HP plus the addition of an improved male Y target for more sensitive male:female ratio detection and improved results in sexual assault casework.

HID Real-Time PCR Analysis Software v1.2 is designed for the 7500 Real-Time PCR System and streamlines the experimental setup, supports customizable workflow templates, and enhances the data analysis and review process. The software is validated for use on both Windows® XP and Windows® 7 platforms, and is specialized for all Quantifiler® kits on the market and optimized for the new Quantifiler® Trio and the Quantifiler® HP DNA Quantification Kits.

Key features and benefits

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Accuracy

More informative and comprehensive casework sample assessment for improved downstream results

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Efficiency

Increased workflow efficiency with faster time-to-results and flexible sample-processing protocols

 
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Flexibility

Flexible protocols allow for configurable output and display features

Decision flow enhancement

The Quantifiler® Trio and Quantifiler® HP kits, used in conjunction with the HID Real-Time PCR Analysis Software, redefine the casework sample-processing workflow. New quality index functionality enables a more comprehensive view of sample quality (both degradation and inhibition), while increased sensitivity and robustness correlate with more recently released Applied Biosystems® STR chemistries. This enables you to screen samples and make critical workflow decisions (for example, autosomal vs. Y-STR) with greater confidence.

The inclusion of quantity and quality indicators helps deliver accurate, sensitive, and specific results that work together with the software to enable informative, integrated workflow decisions.


Solution overview


Enhancing Y targets
Enhancing Genomic Targets
Sensitive and reproducible

Data consistency in Y and genomic targets.

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Software made simple: easy setup for Quantifiler® kits

Standards, targets, run method, and experiment properties are predefined for each Quantifiler® template. Flexibility to support custom assays.

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Data analysis and QC tools

Color-coded analysis summary of standards and samples, hyperlinked to flagged samples for quick data review.

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Webinar and resources

Webinar: Enhancing the Sexual Assault DNA workflow

Learn about powerful new tools for DNA sample assessment and amplification—including the Quantifiler® Trio, Yfiler® Plus, and GlobalFiler® kits—that can help significantly improve the workflow for obtaining interpretable profiles from sexual assault and other challenging casework samples.

View webinar

Development of a Next Generation DNA Quantification Kit with Improved Capabilities and Reduced Time to Result

A. Holt, R. Green, J. Mulero, J. Shewale, J. Muehling

View poster

Enhancing the Sexual Assault Workflow
Enhancing the Sexual Assault Workflow: Testing of Next Generation Assessment and Y-STR Systems

J. Ballantyne, E. Hanson, R. Green, A. Holt and J. Mulero

  View poster (3.7MB)


Automation and HID professional services

As labs worldwide are increasing the throughput of samples through their casework workflows, there is heightened interest in solutions that can increase productivity, maximize data output, and minimize errors.

With our workflow-centric design, we have incorporated robotic system requirements into our latest HID Real-Time PCR Analysis Software v1.2, which enables simple integration and upgrade to a fully optimized, automated quantification system to drive efficiency.

For labs that find it difficult to identify or implement the right robotic system(s) for their midstream workflow, our Human Identification Professional Services (HPS) team has extensive expertise in assisting in the design and implementation of robotic systems from a variety of vendors, and can partner with you to identify the most appropriate solution to meet your specific lab needs.

Product listing

 
Product selection guide
Quantifiler® Trio Quantifiler® HP Quantifiler® Duo Quantifiler® Human Quantifiler® Y
Quantification targets Total human
& male
Total human Total human
& male
Total human Male
Sensitivity <1 pg <1 pg ~6 pg ~16 pg ~16 pg
Quality/
degradation index
Yes Yes No No No
Reaction mix and IPC robustness Excellent Excellent Good Good Good
Correlates with latest STR kits Yes Yes No No No
Accuracy of M:F mixture ratio Excellent N/A Very good N/A N/A
DNA quantification standard Excellent Excellent Very good Good Good
Cycling time ~60 min ~60 min ~90 min ~90 min ~90 min
Minimum standards per plate 5 5 8 8 8
Fully integrated system with instrument, data collection, and analysis software Yes Yes Yes Yes Yes
Validated real-time PCR instrument(s) 7500 7500 7500 7500, 7000 7500, 7000
Compatible analysis software HID Real-Time PCR Analysis Software v1.2 HID Real-Time PCR Analysis Software v1.2 HID Real-Time PCR Analysis Software v1.1, v1.2 or SDS v1.2.3 HID Real-Time PCR Analysis Software v1.1, v1.2 or SDS v1.2.3 HID Real-Time PCR Analysis Software v1.1, v1.2 or SDS v1.2.3
Software with customized workflow templates, data analysis, and dilution/STR setup tools HID Real-Time PCR Analysis Software v1.2 HID Real-Time PCR Analysis Software v1.2 HID Real-Time PCR Analysis Software v1.1, v1.2 HID Real-Time PCR Analysis Software v1.1, v1.2 HID Real-Time PCR Analysis Software v1.1, v1.2
Software with Windows® 7 compatibility and automation/LIMS integration capabilities HID Real-Time PCR Analysis Software v1.2 HID Real-Time PCR Analysis Software v1.2 HID Real-Time PCR Analysis Software v1.2 HID Real-Time PCR Analysis Software v1.2 HID Real-Time PCR Analysis Software v1.2
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