Analyze highly degraded
or trace DNA

The HID-Ion AmpliSeq™ Identity Panel is a ready-to-use panel that includes 124 autosomal markers, and provides discrimination of individuals similar to STR genotype match probabilities used by forensic analysts (between 1 x 10-31 and 6 x 10-35). It's the newer version of our previous HID-Ion AmpliSeq™ Identity Community Panel.

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Single nucleotide polymorphisms (SNPs) provide a variety of new, important information for human identification purposes, in addition to short tandem repeats (STRs). Due to the shorter amplicons required for sequencing, SNPs enable high recovery of information from degraded samples—such as from mass disaster victims.

The ability to multiplex hundreds to thousands of SNP primers, and concurrently amplify and sequence the SNPs, provides significant convenience and high discrimination power comparable to the 13 CODIS core STR loci.

High discrimination power

The ready-to-use HID-Ion AmpliSeq™ Identity Panel, comprising 124 markers, provides discrimination of individuals similar to STR genotype match probabilities used by forensic analysts. High discrimination power is achieved by using 34 upper Y-clade SNPs and 90 autosomal SNPs that have high heterozygosity and low Fixation Index (Fst), described in publications by Kenneth Kidd of Yale University [1] and the SNPforID Consortium [2,3].

This ready-to-use panel version follows a previous HID-Ion AmpliSeq™ Identity Community Panel.

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Fast time-to-results

A simple, modular workflow with minimal hands-on time enables you to quickly go from sample extraction to genotypes and obtain ancestry information in less than 24 hours total (Figure 1).

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Figure 1. HID-Ion AmpliSeq™ Identity Solution workflow. The total estimated 19 hours is based on 8 samples (buccal swabs) on one Ion 314™ Chip. (This workflow does not include either quantification or initialization steps, which are not required for every run.)

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References:

  1. Pakstis AJ, Speed WC, Fang R et al. (2010) SNPs for a universal individual identification panel. Hum Genet 127(3):315–324.
  2. Phillips C, Fang R, Ballard D et al. (2007) Evaluation of the Genplex SNP typing system and a 49plex forensic marker panel. Forensic Sci Int Genet 1(2):180–185.
  3. Karafet TM, Mendez FL, Meilerman MB et al. (2008) New binary polymorphisms reshape and increase resolution of the human Y chromosomal haplogroup tree. Genome Res 18(5):830–838.