Next-generation
punch system technology

The Card Processing Automation 300 (CPA300™) is a fully automated punch instrument designed to process single-source DNA samples on filter paper in forensic and paternity laboratories.

Combining fully automated punching with liquid handling capabilities, the CPA300™ puncher is designed for laboratories interested in improving throughput without compromising sample traceability or data quality.

The CPA200™ puncher is also available, for labs wanting a semi-automated option. (See specifications below.)


The CPA300™ instrument represents the next generation of automated punch systems, and are specifically designed to meet the needs of forensic and paternity laboratories routinely processing single-source samples collected on filter paper. The CPA300™ puncher is a walk-away system that integrates sample card punching, liquid handling, and 96-well plate sealing (also available without the sealing unit) to help reduce the risk of errors and maximize chain of custody. The CPA200™ puncher is a semi-automated benchtop instrument tailored to the needs of low- and medium-throughput laboratories interested in improving efficiency.

The CPA300™ instrument offers:

  • Automatic detection of deposited samples (saliva/blood) using a high-resolution camera and integrated software analysis
  • Camera control performed on each well before and after punching to reduce the risk of errors
  • Traceability of samples through barcode reading
  • User friendly software interface
  • Liquid handling module for automatic dispensing of PCR reagents
  • Reprocessing of already punched positions
  • Integrated sealing unit
Benefit
Punching accuracy
  • Ionizer to remove static
  • Vacuum system to remove debris from punching area
  • Cleaning punch functionality
  • Camera recognition of blood and buccal samples
System flexibility
  • Works with off-the-shelf and custom framed cards
  • Protocol optimized for NUCLEIC-CARD™ with dedicated cleaning area to improve efficiency and reduce time-to-results (only CPA300™)
  • Liquid dispensing unit to dispense PCR reagents for STR amplification (only CPA300™)
  • Plate sealing capabilities to improve efficiency as applicable (only CPA300™)
Full traceability

Plate and card barcode reading

  • Camera control of each well before and after punching
  • Real-time updates on plate status
  • Customizable reports
CPA200™ instrument specifications
Specifications and features1

Supported card formats

All card formats are supported.
The CPA200™ instrument works with sample collection systems routinely used in forensic laboratories, including NUCLEIC-CARD™ (COPAN) FTA® Cards (Whatman) and Buccal DNA Collector devices (Bode), and with both indicating cards (saliva samples) and nonindicating cards (blood samples).

The CPA200™ instrument has the possibility to re-process cards that have already been punched.
Optimized protocol

Using the framed NUCLEIC-CARD™ with cleaning area:
1-spot format: 51 mm x 51 mm

2-spot format: 85 mm x 53 mm

Available punch sizes

The CPA200™ has the following standard punch configurations:
1.2 mm*
2 mm
3 mm
4 mm

*All CPA 200™ instruments come with the1.2 mm punch size by default.

Sample punching position

Framed cards: The user selects the punching position using the external laser pointer. The system will record the coordinates and automatically punch in the selected position.

All other cards: The user selects the punching position using the internal laser pointer aimed at the punching site.

Cleaning procedure

Optimized protocol using the NUCLEIC-CARD™ FRAMED card with cleaning area: First cleaning punch is performed automatically; possibility to repeat for the same cleaning area using the external laser pointer.

All other cards: Cleaning procedure can be performed using a cleaning card or on a blank area of the processing card using the external or internal laser pointer.
Capacity 2 plates (192 cards)

Camera features and imaging

Model: IDS Direct XS
Resolution: 2,592 (h) x 1,944 (v)
Sample presence control: Automatic comparison between the well picture before punching (well normally empty) and after punching (well normally full).

Image storage: All acquired images are saved to a specific directory on the PC desktop.
Physical dimensions 610 mm (L) x 545 mm (W) x 675 mm (H),
Weight: 72 kg

PC Hardware and software

Model: ASEM HT2000-TF/S1: LCD TFT 10.4” 4:3, 10.4” touch-screen, Intel® Atom™ dual core D2550, 1.86 GHz, 1 GB RAM. Operating system: Linux® Ubuntu® .
Peripherals: 10.4’’ touch screen, keyboard, mouse, USB
Communication: USB Data transfer

Network: 2 x Ethernet (1 Gb/s)
LIMS Integration The instrument can be connected to LIMS in order to import and export files in formats suitable for the 3130 and 3500 Genetic Analyzer sequencers. In addition, it can export reports as PDF files.
Main power requirements 400 W (peak) electrical power; 208–240 VAC main supply voltage; frequency 50/60 Hz
Transformers are available for 110V Countries.
Pollution degree 2
Overvoltage category 2

1 Refer to CPA200™ instrument technical documents for additional information on specifications and validated protocols.

CPA300™ instrument specifications:
Specifications and features2

Supported card formats

The CPA300™ instrument works with framed NUCLEIC-CARD™ (COPAN) and FTA® Cards (Whatman) sample collection systems routinely used in forensic laboratories, and both indicating cards (saliva samples) and non-indicating cards (blood samples).
The CPA300™ instrument can reprocess already punched cards.

Optimized protocol

Using the framed NUCLEIC-CARD™ with cleaning area:
1-spot format: 51 mm x 51 mm

2-spot format: 85 mm x 53 mm

Available punch sizes

The CPA300™ instrument has the following standard punch configurations:
1.2 mm*
2 mm
3 mm
4 mm

*All CPA 300™ instruments come with the 1.2 mm punch size by default.

Sample detection

Automatic sample detection (saliva or blood)

Cleaning procedure

Optimized protocol using the NUCLEIC-CARD™ with integrated cleaning area.
Multiple cleaning punches on the same card.

Capacity Fully-automated: 3 plates (288 cards)

Camera features and imaging

Model: Allied Vision Technologies Manta
Resolution: 2452 (h) x 2056 (v)
Sample presence control: Automatic comparison between the well picture before punching (well normally empty) and after punching (well normally full). Verification of the empty/full well before proceeding with the next punching.

Image storage: All acquired images are saved to a specific directory on the PC desktop.
Physical dimensions Including PC: 1,700 mm (L) x 760 mm (W) x 1,005 mm (H)
Operational box: 1,160 mm (L) x 760 mm (W) x 1,005 mm (H)
Weight: 270 kg

PC Hardware and software

The computer is an industrial PC embedded inside the instrument: only the 19"
touch screen, mouse, and keyboard are visible to the user. The computer has a dual
core 2.2 GHz CPU, 4GB RAM and a 32 GB solid state drive. It runs on the Linux® Ubuntu® operating system.
Peripherals: 19'' touch screen/keyboard/mouse/USB (2).
Communication: USB/Data transfer:
Network: Ethernet (1 Gb/s)

LIMS Integration The instrument can be connected to LIMS in order to import and export files
Exportable files
The instrument can export files in formats suitable for the 3130 and 3500 Genetic Analyzer sequencers, and can export reports as PDF files.
Main power requirements 1200W (peak) electrical power; 110-240VAC main supply voltage; 50/60 Hz frequency
Pollution degree 2
Overvoltage category 2
Pipetting Unit 1 pipetting channel
Tip sizes: 50µL, 200µL, 1000µL
Tip station: dedicated area for 3 rack tips
Reagent station: 4 slots with optimized temperature settings

2 Refer to CPA300™ technical documents for additional information on specifications and validated protocols. A version of the CPA300™ without the sealing unit is available.