Anti-GFP-Antibodies_230
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression. We offer anti-GFP antibody conjugates as a rabbit IgG fraction and chicken IgY fraction conjugated to biotin, horseradish peroxidase (HRP), or fluorophore labeled. All anti-GFP antibody conjugates are suited for detection of native GFP, GFP variants, and most GFP fusion proteins by western blot analysis and immunocytochemistry. The anti-GFP rabbit polyclonal antibody is raised against GFP isolated directly from Aequorea victoria and the IgG fraction is purified by ion-exchange chromatography.

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Validated for a variety of applications

  • Western analysis
  • Immunocytochemistry
  • Immunoprecipitation
  • Flow cytometry
  • Immunohistochemistry
Anti-GFP-conjugated_230

Anti-GFP conjugated to Alexa Fluor® 488 with target BacMam-GFP

Alexa Fluor® 488 dye was conjugated to two independent lots of GFP Rabbit IgG Polyclonal antibody resulting in anti-GFP Alexa Fluor® 488 (catalog number A21311). U2OS cells were transduced with CellLight® Mitochondria-GFP, BacMam 2.0 (catalog number C10508).  After an overnight incubation the cells with treated with fixation and then permeablized. GFP molecules were detected by the addition of anti-GFP Alexa Fluor® 488 (catalog number A21311). Texas Red®-X phalloidin (catalog number T7471)  was added to detect actin. Hoeschst 33342 (catalog number H3570) was used as a nuclear counterstain.

Recovering GFP signal after Click-iT ® EdU treatment

Human osteosarcoma cells transduced with CellLight® Mitochondria-GFP (catalog number C10508) samples were pulsed with EdU, fixed, and labeled with Click-iT® EdU Alexa Fluor® 555  (catalog number C10338) for cell proliferation (pink). DAPI for nuclei (blue) (catalog number D3571). GFP signal was recovered with anti-GFP Alexa Fluor® 488 (catalog number A21311) primary antibody (green).

recovering_GFP_230

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Catalog Name Size
A6455 GFP Rabbit Serum Polyclonal Antibody 100 µL
A10262 GFP Chicken IgY Antibody Fraction 100 µL
A11122 GFP Rabbit IgG Polyclonal Antibody Fraction 100 µL
A11120 GFP (clone 3E6) Mouse IgG2a, Monoclonal Antibody 100 µg
G10362 GFP Monoclonal Antibody, ABfinity™ Recombinant 100 µg
R10367 Red Fluorescent Protein (RFP) Rabbit Polyclonal Antibody, Purified 100 µg
A21311 GFP Rabbit IgG Antibody Fraction, Alexa Fluor® 488 Conjugate 100 µL
A31851 GFP Rabbit IgG Antibody Fraction, Alexa Fluor® 555 Conjugate 100 µL
A21312 GFP Rabbit IgG Antibody Fraction, Alexa Fluor® 594 Conjugate 100 µL
A31852 GFP (clone 11E5) Mouse IgG1, Monoclonal Antibody 100 µg