Figure 1:  CD FortiCHO™ medium outperforms various competitive batch culture media with CHOK1SV cell type.
  Cells were passaged at least 12 times for all products for adaptation prior to test culture.  No glutamine was added to cultures.  4 g/L glucose was added whenever glucose levels fell below 2 g/L in culture.  Cultures were grown in 250 mL shake flasks.


Figure 2:  CD FortiCHO™ medium's performance testing (fed-batch).
  CD FortiCHO™ medium with feed outperforms limited glucose fed-batch by nearly 50% in growth and titers achieved with CHOK1SV cell type.  Cells were passaged 2 times following thaw from CD OptiCHO™ freeze prior to test culture.  No glutamine was added to the cultures.  4 g/L glucose was added whenever glucose levels fell below 2 g/L in culture.  Cultures were grown in 125 mL shake flasks.  10% of culture volume was added for EfficientFeed™ supplemented conditions on days 4, 6, 8, and 10.