Simplify downstream protein purification with serum-free CHO Medium

  • Get optimal expression of recombinant proteins
  • Select from chemically defined, protein-free, and serum-free formulations
  • Choose media for suspension cells
  Low-protein (<100 mg/L), serum-free alternative for CHO cell line growth and production Optimizes growth of parental DHFR– CHO cells Consistent production medium High-performing, leaner medium designed for fed-batch optimization Selectively fortified for fastest fed-batch process development starting point
CHO cell line Various CHO dependent on experience DG44, DXB11, or other CHO Various CHO dependent on experience DG44, DXB11, or other CHO CHO K1,
CHO-S®,
GS CHO
Culture type** Suspension Suspension Suspension Suspension Suspension
Classification Serum-Free, contains proteins of animal origin Serum-Free, Protein-Free, Animal Origin-Free, Chemically Defined* Serum-Free, Protein-Free, Animal Origin-Free, Chemically Defined* Serum-Free, Protein-Free, Animal Origin-Free, Chemically Defined* Serum-Free, Protein-Free, Animal Origin-Free, Chemically Defined*
Relative titer in batch culture*** Medium N/A (no titer since cells are parental) High Higher Highest
Culture duration Average <14 days Average <14 days ≥14 days with feeding ≥14 days with feeding ≥20 days with feeding
Recommended Feed CHO CD EfficientFeed™ Kit CHO CD EfficientFeed™ Kit CHO CD EfficientFeed™ B CD EfficientFeed™ A CD EfficientFeed™ C
DMF reference requests Contact Technical Services Contact Technical Services Contact Technical Services Contact Technical Services Contact Technical Services
List price 1L=$87.00 1L=$87.00 1L=$84.00 1L=$76.00 1L=$93.00
Form Liquid Liquid Liquid & AGT™ Liquid & AGT™ Liquid
Liquid sizes 500mL
1L
1L 500ml
1L
10L
20L
1L 1L
AGT™ sizes N/A N/A 1L (24.26g)
10L (242.60g)
100L (2426.0g)
10kg
1L (19.318g)
10L (193.180g)
100L (1931.800g)
10kg
N/A
Option to customize Yes Yes Yes Yes Yes

* Chemically Defined means that the product does not contain any proteins or undefined components like hydrolysates or hydrolysate fractions.
** Products are intended for preparation for and use in stable cell line protein expression. See FreeStyle™ expression products for transient transfection.
*** Dependent on cell line clone