Sample Prep and Automation
AutoMate Express™ Nucleic Acid Extraction System
Time-saving, cost-effective DNA extraction for mycoplasma, MMV, vesivirus, and residual DNA
- Quantitative and reproducible recovery of DNA from complex samples
Out-of-the-box automation with prefilled PrepSEQ® Express cartridges and ready-to-run
- Closed system minimizes the risk of contamination
- Ideal for in-process testing of contaminant and impurities
Sample Prep and Automation
The availability of rapid and accurate testing methods for contaminants and impurities that originate from biopharmaceutical manufacturing processes or from the host cell line is key to enabling in-process testing during manufacturing. The results from those tests can then be used as part of downstream process characterization to make fact-based, go/no-go forward processing decisions.
The AutoMate Express™ Nucleic Acid Extraction System is an easy-to-use benchtop instrument that enables hands-free automation of the nucleic acid extraction process for contaminant and impurity testing. The instrument uses the established PrepSEQ® nucleic acid extraction chemistry for quantitative recovery of DNA and RNA from diverse samples types, including cell culture, raw material (i.e., serum), and in-process downstream purification samples such as column purification or bulk drug substance (BDS) samples.
Three-in-one extraction of cell culture contaminants
The AutoMate Express™ platform can be used to perform nucleic acid extraction of the common contaminants for mammalian cell culture manufacturing, i.e., mycoplasmas, MMV, and vesiviruses. Flexible protocols are available to isolate DNA and RNA from a single organism, or a 3-in-1 surveillance panel protocol can simultaneously extract mycoplasma, MMV, and vesivirus nucleic acid from a single sample. Figures 1 and 2 demonstrate recovery of DNA from spikes of DNA or of cells/viruses of mycoplasmas and MMV into typical cell culture matrices, extracted using the AutoMate Express™ system. The workflow (Figure 3) has been designed for a completely hands-free nucleic acid extraction process enabling reproducible extraction efficiencies and reduced time-to-results.
AutoMate Express™ Nucleic Acid Extraction Systems and Analytical Kits
The AutoMate Express™ platform automatically performs all steps for efficient nucleic acid extraction from contaminants and impurities in a closed system. The system uses PrepSEQ® Express kits that contain sealed cartridges with extraction reagents, and plastics, including tips and sample, lysis, and elution tubes. The small footprint of the instrument enables easy integration into an existing lab setup. Up to 13 samples can be processed typically in under an hour, and the eluates from the extraction can be used directly in real-time PCR-SEQ analytics assays.
Products:AutoMate Express™ Nucleic Acid Extraction System
PrepSEQ® Express Nucleic Acid Extraction Kit