Mycoplasmas, the smallest known free living organisms, are relatively common bacterial contaminants of mammalian cell cultures. Regulatory guidance requires that all products derived from mammalian cell culture be tested for the presence of Mycoplasma.

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Cell culture contaminant detection

MycoSEQ® Mycoplasma Detection System

Dissociation Curves Indicating Positive Results for the Presence of Mycoplasma
 

Mycoplasma detection is difficult, sometimes impossible, when using traditional microbiological techniques. The current Mycoplasma test is a 28-day culture test. In 2007, the European Pharmacopeia accepted nucleic acid tests, such as real-time PCR, as an alternative method for traditional Mycoplasma detection after validation.

Detect Mycoplasma in Hours, Not Days. It's About Time.

 
MycoSEQ® Mycoplasma Detection System
 

The MycoSEQ® Mycoplasma Detection System integrates real-time PCR assays, instruments, and software with optimized PrepSEQ® sample preparation reagents—delivering results in hours, not days. This enables you to test at multiple points in cell lines, cell banks, and harvest media, increasing your productivity and reducing risk of downstream contamination.

The MycoSEQ® Mycoplasma Detection System’s easy workflow delivers actionable results in less than 4 hours—enabling you to detect contamination as early as possible in process development, equipment, raw materials, and finished product.

  • Detection of more than 90 Mycoplasma species
  • Demonstrated sensitivity to less than 10 copies/reaction
  • Proven specificity
  • Proprietary optimized sample preparation for high DNA recovery
  • Externally validated to demonstrate sensitivity of a PCR-based test for Mycoplasma that is not biased toward the success of PCR
  • Seamless integration from detection to identification using the MicroSEQ® Microbial Identification System.

The MycoSEQ™ Mycoplasma Detection System’s easy workflow delivers actionable results typically in less than 4 hours—enabling you to detect contamination as early as possible in process development, equipment, raw materials, and finished product.

Step-by-Step Guide to Mycoplasma Detection Products

Purify DNA from your sample using the PrepSEQ® Mycoplasma Sample Preparation Kit easy magnetic bead-based protocol. Your sample can vary anywhere from 100µl to 10 ml of cell culture. The DNA you purify in this step will be used as your template for Real-Time PCR.

Next, combine the purified DNA template with the assay mix and Power SYBR® Green Master Mix included in the MicroSEQ™ Mycoplasma Detection System.

Next, run your reaction mixture on a Real-Time PCR instrument.

Ordering Information

Sku Name Size Price Qty
4365464 7500 Fast Real-Time PCR System, laptop, QST 1 system Contact Us  

AccuSEQ™ Real-Time PCR Software is an intuitive easy-to-use analysis tool for data interpretation. The software guides the user through the experiment and plate setup and provides easy-to-use tools for defining and setting up standards, controls, and unknowns. AccuSEQ™ v2.0 software is designed for analysis of results obtained from ViralSEQ®, MycoSEQ™, resDNASEQ®, and ProteinSEQ™ kits.

Products

Learn more about AccuSEQ™ v2.0 analysis software

Bioproduction Resources

Have questions about Gibco® media, POROS® chromatography resins, Applied Biosystems® SEQ rapid contaminant tests, or our Cell Therapy products?  Click below to access product brochures, training videos, mobile apps, and more.

 

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