Gibco® EfficientFeed™ + Supplements
Fast, flexible performance in fed-batch cell culture
The power to overcome traditional solubility limitations impacting total bioreactor working volume is now available. With Gibco® EfficientFeed™ A+, B+, and C+ feeding supplements, you can deliver super-concentrated nutrients in a complete, easy-to-prepare, dry-format feed. Maximize without compromise.
- Achieve at least 2- to 5-fold increases over batch culture, with a means to reach higher titers using super-concentration flexibility
- Formulated in Gibco® AGT™ dry format media for simple, rapid preparation that helps reduce risk
- Ability to super-concentrate up to 150–200g/L at neutral pH, without pH adjustment
- Room temperature storage of reconstituted liquid during a run means fewer closed-system connections
- Single-part product means fewer steps in reconstitution
Ultimate flexibility through super-concentration
The EfficientFeed™ + Supplements are designed with the flexibility to super-concentrate chemically defined components up to 3X (i.e., 200 g/L) of original levels while remaining pH-neutral. This is accomplished with nutrient delivery technology that allows reconstitution without pH adjustments (i.e., just add water). By adding different amounts of water during reconstitution, you can concentrate these supplements 2X, or up to 3X, as illustrated below.
Comparison of final volumes for reconstituting a 10 L package of EfficientFeed™ + Supplement.
How to super-concentrate
- Weigh out the increased amount indicated here instead of the 1X amount.
- Mix for the extended time shown here to help completely dissolve the more concentrated supplements.
|EfficientFeed™ A+||1X (66.4 g/L)||20 min|
|3X (199.2 g/L)||75 min|
|EfficientFeed™ B+||1X (52.7 g/L)||20 min|
|3X (158.1 g/L)||50 min|
|EfficientFeed™ C+||1X (81.2 g/L)||40 min|
|2X (162.4 g/L)||60 min|
Traditionally, bioreactor space necessitated a compromise: the amount of complete feeding supplements one could add meant a trade-off between initial working volume and feeding space (dilution factor).
EfficientFeed™ + Supplements enable you to deliver up to 200 g/L of needed nutrients in a complete, super-concentrated, pH-neutral feed. The reduction in required feeding space provides you with three ways to optimize your bioreactor output for every run:
- Reduce product dilution
- Maximize working volume
- Increase feeding of nutrients for increased titer
|Product||Concentration||% Addition on Culture Days Indicated|
|EfficientFeed™ A+||1X (66.4 g/L)||10%||-||10%||-||10%||-||10%|
|3X (199.2 g/L)||3.3%||-||3.3%||-||3.3%||-||3.3%|
|EfficientFeed™ B+||1X (52.7 g/L)||10%||-||10%||-||10%||-||10%|
|3X (158.1 g/L)||3.3%||-||3.3%||-||3.3%||-||3.3%|
|EfficientFeed™ C+||1X (81.2 g/L)||10%||-||10%||-||10%||-||10%|
|2X (162.4 g/L)||5%||-||5%||-||5%||-||5%|
We made it even better
Gibco® EfficientFeed™ + Supplements are designed to provide the same 2- to 5-fold increases in titer possible with original EfficientFeed™ supplements, plus the flexibility to be super-concentrated up to 3X (or 200 g/L) of original levels while remaining pH-neutral and chemically defined.
In the figure below, we demonstrate an improvement in titer with super-concentrated EfficientFeed™ C+ supplement.
Titers of CHO DG44 cells in CD FortiCHO™ medium fed with EfficientFeed™ C (EFC) or EfficientFeed™ C+ (EFC+) supplement.
Gibco® EfficientFeed™ + Supplements work even better than the original versions. But how do they perform against other supplements?
The quality you expect from Gibco® products
Consistent protein quality delivered.
With EfficientFeed™ + Supplements, performance doesn’t stop at titer. Product quality is also a critical parameter.
Here we examined the impact of changing from EfficientFeed™ C to EfficientFeed™ C+ Supplement on the glycoforms of a target protein and saw no change in protein quality, even when EfficientFeed™ C+ was super-concentrated.
Simple preparation helps reduce risk
Our Advanced Granulation Technology™ dry media format provides ease of use while helping to reduce risk by minimizing the steps and adjustments needed to prepare media.
Storage of reconstituted liquid also reduces risk
Typical Closed Systems Connections Required
The ability to store reconstituted EfficientFeed™+ Supplements at room temperature, in the dark, for the period of a typical fed-batch bioreactor run adds flexibility to make just one closed-system connection per bioreactor run instead of multiple closed-system connections. This reduces the opportunities for an error that could lead to contamination of a run.
Caution: For manufacturing, processing, or repacking.