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Achieve Right the First Time

Used in top pharmaceutical companies worldwide, the Life Technologies MicroSEQ Rapid Microbial Identification System is ideal for environmental monitoring, contamination investigation, root cause analysis, raw materials testing, and microbial identification in small molecule and biopharmaceutical manufacturing, and service laboratories. It can also be used in public health laboratories, cosmetics and personal care products manufacturing, food testing laboratories, and academic and research centers.

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Leaving microbial ID to chance?

Why is Microbial Identification Important?

Harmful bacteria and fungi can contaminate raw materials, production facilities, and branded products. Regulatory expectations include accurate identification of environmental isolates to support active environmental monitoring programs within pharmaceutical manufacturing environments as part of cGMP, and to implement accurate Corrective and Preventive Actions (CAPAs) for products and processes to reduce the risk of contamination. The US, European, and Japanese Pharmacopeia guidelines recommend genotypic microbial identification of bacteria and fungi at the species level. The comparative gene sequence analysis of ribosomal DNA (rDNA) has been shown to have the highest accuracy of microbial identification system technologies and has been considered the gold standard for microbial identification for over a decade.

Where to test for Bacteria & Fungi?

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MicroSEQ® Rapid Microbial Identification System Featuring the 3500 Genetic Analyzer

Go Molecular. The Answer Is in the DNA

It's time to take microbial identification to the next level. It's time to partner with the proven leader in rapid molecular methods. It's time for the MicroSEQ® Rapid Microbial Identification System in your QC Microbiology lab. Built on years of molecular expertise, this system delivers unparalleled accuracy and time to results for bacterial and fungal identifications in one easy-to-use platform.

  • Accurate genotypic bacterial identification based on the 16S rRNA gene
  • Accurate genotypic fungal identification based on the D2 region of the 26S rRNA gene
  • Easy workflow, high throughput, accurate results typically in less than five hours
  • Fully validated and implemented in as few as four months
  • Enables 21 CFR Part 11 compliance

 

Step-by-Step Guide to Microbial Identification & Detection Products

Extract DNA from bacteria or fungi using the PrepMan ® Ultra sample preparation reagent. Your sample can be a single colony or a pure culture. The ideal colony size is 1-2 mm for bacteria and 3 mm for fungi. The DNA you extract in this step will be your template for the PCR step.

Ordering Information

Sku Name Size Price Qty
4318930 PrepMan® Ultra Sample Preparation Reagent 1 kit USD 121.00
4322547 PrepMan® Ultra Sample Preparation Reagent, with protocol 1 kit USD 129.00

Use the MicroSEQ ® Microbial PCR Kit that matches .your needs. Each kit contains reagents for amplification (PCR) reactions. Run the reactions on the Veriti 96-Well thermal cycler. Clean up the PCR products for the subsequent step using the MicroSEQ® clean-up kits.

Use the MicroSEQ ® Microbial Sequencing Kit that matches .your needs. Each kit contains reagents for cycle sequencing reactions. Run the reactions on the Veriti 96-Well thermal cycler. Clean up the products for the subsequent steps using the MicroSEQ® Clean-up kits. Next, sequence your sample automatically by running it on a genetic analyzer (DNA seqeuncer). Choose the system configuration that meets your throughput needs.

Ordering Information

Sku Name Size Price Qty
4346480 MicroSEQ® 500 16S rDNA Sequencing Kit 1 kit USD 402.77
4346479 MicroSEQ®500 16S rDNA Sequencing Kit, with protocol 1 kit USD 408.87
4347484 MicroSEQ® Full Gene 16S rDNA Sequencing Kit 1 kit USD 689.59
4347483 MicroSEQ® Full Gene 16S rDNA Sequencing Kit, with protocol & quick reference card 1 kit USD 750.62
4347481 MicroSEQ® D2 rDNA Fungal Sequencing Kit 1 kit USD 545.17
4347482 MicroSEQ® D2 rDNA Fungal Sequencing Kit, with protocol & quick reference card 1 kit USD 598.05
4415506 MicroSEQ® ID; ID Clean-Up Kit 1 kit USD 470.92
4427807 MicroSEQ® ID; Combination Kit, with Clean-Up Plates 1 kit USD 252.24
4440462 3500 Genetic Analyzer for Resequencing & Fragment Analysis 1 system Request a Quote  

Use the MicroSEQ® ID Analysis Software to match sequences in your sample with sequences in our validated libraries. The software also enables you to create custom libraries and search public libraries.

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MicroSEQ® ID Analysis Software

Ordering Information

Sku Name Size Price Qty
4448336 MicroSEQ® ID Microbial Identification Software v3.0 Bundle for 3500 Instrument 1 kit USD 34,608.00
4467661 MicroSEQ® ID Microbial Identification Software v3.0 Bundle for 3500 Instrument (3 licenses) 1 kit USD 56,650.00
4467862 MicroSEQ® ID Microbial Identification Software v3.0 Lite Bundle 1 kit USD 25,750.00
4467863 MicroSEQ® ID Microbial Identification Software v3.0 Lite Bundle (3 licenses) 1 kit USD 41,972.00
4398220 MicroSEQ® ID 16S rDNA 500 Library v2013 1 kit USD 10,825.00
4371296 MicroSEQ® ID Fungal Gene Library v2013 1 kit USD 10,825.00

IQ/OQ Service
Overcome critical implementation challenges in the qualification of instrument and operation quality (IQ/OQ) through a collaborative partnership with Applied Biosystems.

  • Consultation with client to determine needs
  • Statement of work to define project
  • Detailed verification test plan and documentation
  • System installation verification
  • Sequence of system operations qualification
  • Microbial identification verification
  • Accuracy report
  • System software installation and security verification
  • Software exporting, importing, archiving and verification
  • Phylogenetic tree and custom library verifications
  • Detailed data analysis and review
  • Consultation on experimental process and results
  • Performance Qualification (PQ) recommended guidelines

Implementation Program
Customize laboratory design and training to the specific needs of your lab, with the aim of completing Performance Qualification (PQ) within the six-month timeframe of the program.

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Positive Financial Impact

Would you like an ROI assessment of your current system versus MicroSEQ Rapid? Contact your local sales representative for a review.

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Bioproduction Resources

Have questions about Gibco® media, POROS® chromatography resins, Applied Biosystems® SEQ rapid contaminant tests, or our Cell Therapy products?  Click below to access product brochures, training videos, mobile apps, and more.

 

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